The Determination of Esterified Fatty Acids in Glycerides, Cholesterol Esters and Phosphatides PDF Download
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Author: W. D. Skidmore
Publisher:
ISBN:
Category : Cholesterol
Languages : en
Pages : 54
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Book Description
The hydroxamic acid reaction conditions for the determination of esterified fatty acids were modified to the extent that the variable factors involved were controlled so that the molar absorptivities per ester group for triglycerides, cholesteryl esters of long-chain fatty acids, and phosphatides were equivalent through 8 microequivalent ester. The amount of water present during the formation of hydroxamates was the most important single factor in obtaining equivalent color values with these 3 types of esters. The accuracy and precision of the method were well defined by showing that the optical density values for 5 different ester standards were on the identical straight line curve. Spectral curves between the wavelengths of 410 and 700 millimicron with standard carboxylic acid esters and Folch extracts of rat serum, rat liver, and human serum were qualitatively and quantitatively identical. A long chain cholesteryl ester must be used as one of the standard esters because of its solubility characteristics and water sensitivity. Cholesteryl acetate can not be used as a reliable representative in place of a long chain cholesteryl ester. (Author).
Author: W. D. Skidmore
Publisher:
ISBN:
Category : Cholesterol
Languages : en
Pages : 54
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Book Description
The hydroxamic acid reaction conditions for the determination of esterified fatty acids were modified to the extent that the variable factors involved were controlled so that the molar absorptivities per ester group for triglycerides, cholesteryl esters of long-chain fatty acids, and phosphatides were equivalent through 8 microequivalent ester. The amount of water present during the formation of hydroxamates was the most important single factor in obtaining equivalent color values with these 3 types of esters. The accuracy and precision of the method were well defined by showing that the optical density values for 5 different ester standards were on the identical straight line curve. Spectral curves between the wavelengths of 410 and 700 millimicron with standard carboxylic acid esters and Folch extracts of rat serum, rat liver, and human serum were qualitatively and quantitatively identical. A long chain cholesteryl ester must be used as one of the standard esters because of its solubility characteristics and water sensitivity. Cholesteryl acetate can not be used as a reliable representative in place of a long chain cholesteryl ester. (Author).
Author:
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 216
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Author: National Research Council
Publisher: National Academies Press
ISBN: 0309040418
Category : Medical
Languages : en
Pages : 298
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Book Description
Since its introduction in 1943 Recommended Dietary Allowances has become the accepted source of nutrient allowances for healthy people. These Recommended Dietary Allowances (RDAs) are used throughout the food and health fields. Additionally, RDAs serve as the basis for the U.S. Recommended Daily Allowances, the Food and Drug Administration's standards for nutrition labeling of foods. The 10th Edition includes research results and expert interpretations from years of progress in nutrition research since the previous edition and provides not only RDAs but also "Estimated Safe and Adequate Daily Dietary Intakes"â€"provisional values for nutrients where data were insufficient to set an RDA. Organized by nutrient for ready reference, the volume reviews the function of each nutrient in the human body, sources of supply, effects of deficiencies and excessive intakes, relevant study results, and more. The volume concludes with the invaluable "Summary Table of Recommended Dietary Allowances," a convenient and practical summary of the recommendations.
Author: W. D. Skidmore
Publisher:
ISBN:
Category : Chromatographic analysis
Languages : en
Pages : 40
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Author: Hans-UIrich Bergmeyer
Publisher: Elsevier
ISBN: 0323161324
Category : Science
Languages : en
Pages : 800
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Book Description
Methods of Enzymatic Analysis, Volume 4 reviews developments in the use of enzymes as tools in analytical biochemistry, including advances in assay techniques. It discusses the principles and methods for the elucidation of structures of enzymes, such as peptides, proteins, amino acids, fatty acid metabolites, lipids, steroids, nucleic acids, purines, pyrimidines, nucleosides, and coenzymes. It also considers the isolation and characterization of active centers in enzymes. This volume is divided into four parts, each discussing a group of enzymes and their determination. Part I focuses on proteins, peptides, and amino acids including amines and amides. Part II is concerned with fatty acid metabolites, lipids, and steroids ranging from polyunsaturated fatty acids and lecithin to choline, acetylcholine, triglycerides, glycerol, acetoacetate, triacetate, fumarylacetoacetate, 20-ketosteroids, prostaglandins, bile acids, and cholesterol. Part III discusses nucleic acids, purines, pyrimidines, nucleosides, coenzymes, and related compounds, whereas Part IV looks at other substrates and effectors such as inorganic phosphate. The book concludes with a chapter on metabolites and their concentrations in animal tissues. Biochemists as well as students and researchers working in the field of analytical biochemistry will find this book highly informative.
Author: Edwin Daniel
Publisher: Springer Science & Business Media
ISBN: 1468427512
Category : Medical
Languages : en
Pages : 712
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Book Description
The study of the actions of drugs on smooth muscle has been a preoccupation of many pharmacologists almost from the beginning of the discipline. To a con siderable degree, the development of theories to explain drug actions on smooth muscle has occurred somewhat independently of the development of our knowledge of the physiology, biochemistry, and biophysics of smooth muscle. This knowledge has developed rapidly in the past decade, and some of its consequences for our understanding of drug-receptor interactions in smooth muscle have not always been fully appreciated or accepted. One of the purposes of this volume is to provide pharmacologists with some understanding of the physiology, biophysics, and bio chemistry of smooth muscle and of related advances in methodology so as to facilitate the incorporation of such knowledge and related methods into future pharmacological studies of smooth muscle and drug interactions. Another purpose of the book is to provide both graduate students and in vestigators in pharmacology and related disciplines with a summary of the numerous methods that have evolved or are available for the study of drug and smooth muscle interactions, and, in particular, to highlight their possible uses and limitations. Perhaps, because of the diversity in content and difficulty of these methods, there has to our knowledge never been a previous attempt to bring them together in one place. We have not, of course, succeeded entirely in this objective.
Author: Anil Gupta
Publisher: Springer
ISBN: 9811310351
Category : Medical
Languages : en
Pages : 604
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Book Description
This book combines fundamental concepts of biochemistry and the dental sciences to provide an authentic, coherent and comprehensive text for dental students. It describes in simple language the intricate pathophysiology of biomolecules in health and in diseases of dental and oral tissues. This book also describes the evolution of biochemistry in a chronological order, provides information about the fundamental chemical structure, classification and biological significance of biomolecules, vitamins and hormones, enriched with flow charts and diagrams for easy understanding and quick reference. It includes chapters on nucleic acids, nutrition and serum enzymes and organ function tests, and offers an innovative approach to familiarize dental students with the biochemical composition of enamel, dentine, cementum and saliva, explaining the biochemical basis of dental caries, periodontal diseases, role of fluorides in caries prophylaxis, fluoride toxicity, and the role of amino acids as anti-hypersensitive agents.
Author:
Publisher:
ISBN:
Category : Anticoagulants (Medicine)
Languages : en
Pages : 1352
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Author: Wesley Dean Skidmore
Publisher:
ISBN:
Category :
Languages : en
Pages : 194
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Author:
Publisher:
ISBN:
Category : Medicine
Languages : en
Pages : 1364
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