Testing Methods for Seed-transmitted Viruses

Testing Methods for Seed-transmitted Viruses PDF Author: Sven Erik Albrechtsen
Publisher: CABI
ISBN: 0851990169
Category : Science
Languages : en
Pages : 280

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Book Description
This book provides a practical guide to the commonly used detection methods for seed-transmitted viruses and viroids affecting both tropical and non-tropical crops. The first part describes important aspects of seed-transmitted viral diseases. The second and main part contains principles of the detection techniques and step-by-step protocols accompanied by method optimization and comments. Most of the described techniques can be equally applied to plant viruses and viroids other than seedborne ones. This book will be of significant interest to those working in seed testing laboratories and students and teachers within plant pathology and seed science.

Testing Methods for Seed-transmitted Viruses

Testing Methods for Seed-transmitted Viruses PDF Author: Sven Erik Albrechtsen
Publisher: CABI
ISBN: 0851990169
Category : Science
Languages : en
Pages : 280

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Book Description
This book provides a practical guide to the commonly used detection methods for seed-transmitted viruses and viroids affecting both tropical and non-tropical crops. The first part describes important aspects of seed-transmitted viral diseases. The second and main part contains principles of the detection techniques and step-by-step protocols accompanied by method optimization and comments. Most of the described techniques can be equally applied to plant viruses and viroids other than seedborne ones. This book will be of significant interest to those working in seed testing laboratories and students and teachers within plant pathology and seed science.

Testing Methods for Seed-transmitted Viruses

Testing Methods for Seed-transmitted Viruses PDF Author: S. E. Albrechtsen
Publisher: CABI
ISBN: 1845931483
Category : Science
Languages : en
Pages : 282

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Book Description
This practical guide covers the commonly used detection methods for seed-transmitted viruses and viroids that affect both tropical and temperate crops. It contains 25 complete step-by-step procedures for biological, serological and molecular techniques to detect and identify such viruses. Combining helpful practical notes with more detailed explanations of the principles behind the techniques, the book describes the general characteristics of seed-transmitted viral diseases and discusses outlines for the organization and interpretation of seed health assays. The techniques reviewed are also applicable to non-seed-transmitted viral agents.

Seed-borne plant virus diseases

Seed-borne plant virus diseases PDF Author: K. Subramanya Sastry
Publisher: Springer Science & Business Media
ISBN: 8132208137
Category : Medical
Languages : en
Pages : 328

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Book Description
Seeds provide an efficient means in disseminating plant virus and viroid diseases. The success of modern agriculture depends on pathogen free seed with high yielding character and in turn disease management. There is a serious scientific concern about the transmission of plant viruses sexually through seed and asexually through plant propagules. The present book provides the latest information along with the total list of seed transmitted virus and viroid diseases at global level including, the yield losses, diagnostic techniques, mechanism of seed transmission, epidemiology and virus disease management aspects. Additional information is also provided on the transmission of plant virus and virus-like diseases through vegetative propagules. It is also well known that seed transmitted viruses are introduced into new countries and continents during large-scale traffic movements through infected germplasm and plant propogules. The latest diagnostic molecular techniques in different virus-host combinations along with disease management measures have been included. The book shall be a good reference source and also a text book to the research scientists, teachers, students of plant pathology, agriculture, horticulture, life sciences, green house managers, professional entrepreneurs, persons involved in quarantines and seed companies. This book has several important features of seed transmitted virus diseases and is a good informative source and thus deserves a place in almost all university libraries, seed companies and research organizations.

Seed-Borne Diseases of Agricultural Crops: Detection, Diagnosis & Management

Seed-Borne Diseases of Agricultural Crops: Detection, Diagnosis & Management PDF Author: Ravindra Kumar
Publisher: Springer Nature
ISBN: 9813290463
Category : Technology & Engineering
Languages : en
Pages : 860

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Book Description
The global population is increasing rapidly, and feeding the ever-increasing population poses a serious challenge for agriculturalists around the world. Seed is a basic and critical input in agriculture to ensure global food security. Roughly 90 percent of the crops grown all over the world are propagated by seed. However, seed can also harbour and spread pathogens, e.g. fungi, bacteria, nematodes, viruses etc., which cause devastating diseases. Seed-borne pathogens represent a major threat to crop establishment and yield. Hence, timely detection and diagnosis is a prerequisite for their effective management. The book "Seed-Borne Diseases of Agricultural Crops: Detection, Diagnosis & Management" addresses key issues related to seed-borne/transmitted diseases in various agricultural crops. Divided into 30 chapters, it offers a comprehensive compilation of papers concerning: the history of seed pathology, importance of seed-borne diseases, seed-borne diseases and quarantine, seed health testing and certification, detection and diagnosis of seed-borne diseases and their phytopathogens, host-parasite interactions during development of seed-borne diseases, diversity of seed-borne pathogens, seed-borne diseases in major agricultural crops, non-parasitic seed disorders, mechanisms of seed transmission and seed infection, storage fungi and mycotoxins, impact of seed-borne diseases on human and animal health, and management options for seed-borne diseases. We wish to thank all of the eminent researchers who contributed valuable chapters to our book, which will be immensely useful for students, researchers, academics, and all those involved in various agro-industries.

A Manual of Rice Seed Health Testing

A Manual of Rice Seed Health Testing PDF Author: T. W. Mew
Publisher: Int. Rice Res. Inst.
ISBN: 9712200493
Category : Technology & Engineering
Languages : en
Pages : 121

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Book Description
Rice seed health and quarantine; The rice plant and its environment; Equipment; Samples and sampling; dry seed inspection; Fungi; Bacteria; Nematodes; Viruses and mycoplasmalike organisms; Field inspection; Seed treatment; Weed seed; Insect pests; Fungal pathogens; Bacterial pathogens; Nematode pest; Organisms causing grain discoloration and damage.

Plant Virus and Viroid Diseases in the Tropics

Plant Virus and Viroid Diseases in the Tropics PDF Author: K. Subramanya Sastry
Publisher: Springer Science & Business Media
ISBN: 940076524X
Category : Science
Languages : en
Pages : 389

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Book Description
Plant virus and sub-viral pathogens pose severe constraints to the production of wide range of economically important crops worldwide. The crops raised both through true seed and vegetative propagated materials are affected with number of virus and virus-like diseases. The virus may enter into plants through seed planting materials or by vectors. Once the virus is in the field, it multiplies and spreads following definite patterns depending upon the nature of the vector and agro-meteorological conditions. Disease free crops and plants are great economic and social importance in feeding the world's population. Detection of virus and sub-viral agents at initial stages of infection is critical to reduce economic losses. For nearly two decades, ELISA and its variants played a major role in large scale virus testing and also in the production of virus-free planting materials.

Seed Pathology

Seed Pathology PDF Author: Paul Neergaard
Publisher:
ISBN:
Category : Pathology
Languages : en
Pages : 878

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Book Description
V.1: Pathognes-diseases-hosts; Mechanisms of seed transmission; Principles of control; Seed health testing methods; Assessment of seed-borne inoculum. v. 2: Pathogens-diseases-hosts; Mechanisms of seed transmission; Principles of control; Seed health testing methods; Assessment of seed-borne inoculum.

Plant Viruses and Virus Diseases

Plant Viruses and Virus Diseases PDF Author: F.C. Bawden
Publisher: Daya Books
ISBN: 9788176220644
Category : Science
Languages : en
Pages : 360

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Book Description
The Subject Of Plant Viruses And Virus Diseases Are Receiving Increasing Attention At The Hands Of Research Scholars Such As Chemists, Crystallographers, Entomologists, Geneticists, Serologists, Physicists Etc. They Are Able To Bring Fresh Techniques To The Subject. The Book Is An Attempt To Describe And Correlate The Advances That Have Been Made So Far In The Study Of Plant Viruses, And Virus Diseases. During The Past Few Years, Many New Virus Diseases Have Been Described, Some Of Great Economic Importance And Some With Novel Kinds Of Symptoms; Much Has Been Done On The Factors That Affect Spread; And Insect Vectors Have Been Identified In Species Of Insects Previously Not Incriminated. More Viruses Have Been Purified And Their Chemical Constitution Has Been Further Elucidated, And The Advances In Electron Microscopy Have Provided Much Information On Their Morphology. All These Developments Have Been Incorporated In The Book. The Book Is A Treasure For Agriculture And Horticulture Departments And All Those Interested In These Subjects. Contents Chapter 1: Introduction; Definition Of Subject, Economic Importance, Historical, Views On The Nature Of Viruses, Nomenclature, Chapter 2: External Symptoms Of Infected Plants; Range Of Host Plant Reactions, Type Diseases, Virus Strains, Effects Of Environment On Symptoms, Chapter 3: Internal Symptoms Of Infected Plants; Intracellular Inclusions Formed By Tobacco Mosaic Viruses, Possible Relationship Between Tobacco Mosaic Viruses And The Intracellular Inclusions, Intracellular Inclusions Caused By Viruses Other Than Tobacco Mosaic, Internal Changes Other Than Intracellular Inclusions, Chapter 4: Methods Of Transmission; Grafting And The Use Of Dodder, Inoculation With Plant Extracts, Transmission By Insects, Seed Borne Viruses, Soil Borne Viruses, Chapter 5: The Relations Between Viruses And Their Vectors; Viruses Which Persist In Their Insect Vectors, Latent Period And Virus Multiplication In Vectors, Viruses Whose Vectors Soon Lose Infectivity, Effect Of The Number Of Insects In Transmitting, Chapter 6: Mutation, Interference Between Strains, And Recovery From Disease; Kinds Of Variation, Origin Of Variation, Acquired Immunity, Chapter 7: Serological Reactions Of Plant Viruses; Preparation Of Antisera, Methods Of Testing, The Antigenicity Of Plant Viruses, Neutralisation Of Infectivity, Specificity Of Serological Reactions, Effect Of Particle Shape Of Serological Reactions, Chapter 8: Quantitative Methods Of Assaying For Viruses; The Use Of Local Lesions, Starch Iodine Lesions, The Use Of Preciptin Tests, Chapter 9: The Purification Of Viruses; Treatment Of Plants, Tobacco Mosaic And Other Anisometric Viruses, Tomato Bushy Stunt And Other Crystalline Viruses, High Speed Centrifugation, Activity Of Purifie Preparations, Proteins From Healthy Plants, Chapter 10: Chemical And Physical Properties Of Purified Virus Preparations; Elementary Composition, Isolation And Properties Of Nucleic Acid, The Denatured Protein, Specific Volume And Hydration, Precipitation And Isoelectric Points, Chapter 11: The Crystallinity Of Plant Viruses; Optical Properties Of Crystals, Anistropy Of Flow And The Layering Phenomenon, Crystal And Liquid Crystals, Chapter 12: The Estimation Of Particle Sizes; Filtration, Sedimentation And Diffusion, Microscopy And X-Ray Diffraction, Additional Methods, The Aggregation Of Anisometric Viruses, Chapter 13: Types Of Inactivation; Inhibition Of Infectivity, Inactivation Without Lossof Serological Activity, Inactivation And Denaturation, Chapter 14: Taxonomy Of Viruses; Nomenclature And Classification, The Grouping Of Virus Strains, The Formation Of Larger Groups, Chapter 15: Virus Diseases And Host Plant Physiology; Host Plant Metabolism And Susceptibility, Effects Of Viruses On Host Plant Metabolism, The Movement Of Viruses, Chapter 16: Control Measures Against Virus Diseases; Curative Treatments, General Measures, Insect Borne Viruses, Viruses Transmitted Mechanically, Chapter 17: Speculations On The Origins Of Viruses.

Characterization of Plant Viruses

Characterization of Plant Viruses PDF Author: Alangar Ishwara Bhat
Publisher: Humana
ISBN: 9781071603369
Category : Science
Languages : en
Pages : 552

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Book Description
This book provides detailed information on methodologies used in biological, serological and nucleic acid based assays for the detection, diagnosis and management of plant viruses. The content is divided into six main parts, the first of which presents techniques used in the biological characterization and transmission of viruses, while Part II covers purification and techniques concerning the physico-chemical properties of viruses. In turn, Part III focuses on in vitro expression, production of polyclonal and monoclonal antibodies; and on various serological assays including precipitin tests, ELISA, blot immunoassays, immunosorbent electron microscopy and lateral flow immunoassays. Part IV addresses the isolation of DNA and RNA from plants and nucleic acid based assays such as dot-blot, polymerase chain reaction, real-time PCR, loop-mediated isothermal amplification, rolling circle amplification, recombinase polymerase amplification, and next-generation sequencing approaches. Part V discusses cloning, sequencing, sequence analysis and the production of infectious clones, while the last part (Part VI) provides biotechnological approaches for the management of viruses. Given its scope, the book will be a valuable resource for every laboratory, student and teacher, and for everyone interested in plant virology, plant pathology, plant biology and molecular biology, offering them a practical manual on various aspects of plant viruses.

Seed Transmitted Virus Diseases

Seed Transmitted Virus Diseases PDF Author: Lene Lange
Publisher:
ISBN: 9789576162374
Category : Seed pathology
Languages : en
Pages : 165

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Book Description