Theory and Practice of Scanning Optical Microscopy PDF Download
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Author: Tony Wilson
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 228
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Book Description
Author: Tony Wilson
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 228
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Book Description
Author: Timothy Richard Corle
Publisher:
ISBN:
Category :
Languages : en
Pages : 298
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Book Description
Author: Gordon S. Kino
Publisher: Academic Press
ISBN: 9780080529783
Category : Technology & Engineering
Languages : en
Pages : 335
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Book Description
This book provides a comprehensive introduction to the field of scanning optical microscopy for scientists and engineers. The book concentrates mainly on two instruments: the Confocal Scanning Optical Microscope (CSOM), and the Optical Interference Microscope (OIM). A comprehensive discussion of the theory and design of the Near-Field Scanning Optical Microscope (NSOM) is also given. The text discusses the practical aspects of building a confocal scanning optical microscope or optical interference microscope, and the applications of these microscopes to phase imaging, biological imaging, and semiconductor inspection and metrology.A comprehensive theoretical discussion of the depth and transverse resolution is given with emphasis placed on the practical results of the theoretical calculations and how these can be used to help understand the operation of these microscopes. Provides a comprehensive introduction to the field of scanning optical microscopy for scientists and engineers Explains many practical applications of scanning optical and interference microscopy in such diverse fields as biology and semiconductor metrology Discusses in theoretical terms the origin of the improved depth and transverse resolution of scanning optical and interference microscopes with emphasis on the practical results of the theoretical calculations Considers the practical aspects of building a confocal scanning or interference microscope and explores some of the design tradeoffs made for microscopes used in various applications Discusses the theory and design of near-field optical microscopes Explains phase imaging in the scanning optical and interference microscopes
Author: Josef F. Bille
Publisher: Springer
ISBN: 3030166384
Category : Medical
Languages : en
Pages : 407
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Book Description
This open access book provides a comprehensive overview of the application of the newest laser and microscope/ophthalmoscope technology in the field of high resolution imaging in microscopy and ophthalmology. Starting by describing High-Resolution 3D Light Microscopy with STED and RESOLFT, the book goes on to cover retinal and anterior segment imaging and image-guided treatment and also discusses the development of adaptive optics in vision science and ophthalmology. Using an interdisciplinary approach, the reader will learn about the latest developments and most up to date technology in the field and how these translate to a medical setting. High Resolution Imaging in Microscopy and Ophthalmology – New Frontiers in Biomedical Optics has been written by leading experts in the field and offers insights on engineering, biology, and medicine, thus being a valuable addition for scientists, engineers, and clinicians with technical and medical interest who would like to understand the equipment, the applications and the medical/biological background. Lastly, this book is dedicated to the memory of Dr. Gerhard Zinser, co-founder of Heidelberg Engineering GmbH, a scientist, a husband, a brother, a colleague, and a friend.
Author: Tony Wilson
Publisher:
ISBN:
Category : Microscope and microscopy
Languages : en
Pages : 232
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Book Description
Author: Jens Rietdorf
Publisher: Springer Science & Business Media
ISBN: 9783540236986
Category : Science
Languages : en
Pages : 344
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Book Description
With contributions by numerous experts
Author: James Pawley
Publisher: Springer Science & Business Media
ISBN: 1461571332
Category : Medical
Languages : en
Pages : 234
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Book Description
In 1987 the Electron Microscopy Society of America (EMSA) going to drive important scientific discoveries across wide areas under the leadership of J. P. Revel (Cal Tech) initiated a major of physiology, cellular biology and neurobiology. They had been program to present a discussion of recent advances in light looking for a forum in which they could advance the state of microscopy as part of the annual meeting. The result was three the art of confocal microscopy, alert manufacturers to the lim special LM sessions at the Milwaukee meeting in August 1988: itations of current instruments, and catalyze progress toward The LM Forum, organized by me, and Symposia on Confocal new directions in confocal instrument development. LM, organized by G. Schatten (Madison), and on Integrated These goals were so close to those of the EMSA project that Acoustic/LM/EM organized by C. Rieder (Albany). In addition, the two groups decided to join forces with EMSA to provide there was an optical micro-analysis session emphasizing Raman the organization and the venue for a Confocal Workshop and techniques, organized by the Microbeam Analysis Society, for NSF to provide the financial support for the speakers expenses a total of 40 invited and 30 contributed papers on optical tech and for the publication of extended abstracts.
Author: Michael Johannes Nasse
Publisher:
ISBN:
Category :
Languages : en
Pages : 212
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Book Description
We present the development and application of a hybrid confocal and aperture near-field scanning optical microscope operating in reflection mode. It has been designed to work at cryogenic temperatures and in vacuum or with an exchange gas. Furthermore, it permits to split the fluorescence into two perpendicular polarization channels.We have studied various single nano-objects, such as molecules, CdSe/ZnSe nanocrystals, CdSe/ZnS nanorods and fluorescent nanospheres. As molecular samples we used diI-C18 molecules and molecules derived from anthracene. The ensemble absorption and emission spectra of the latter were measured, as well as their absorption cross-section and quantum yield in solution for the first time. Furthermore, we used time correlated fluorescence decay experiments that suggest the presence of different conformations in solution, already observed with similar molecules.We used our instrument in confocal mode to image, record time traces, and acquire some spectra of single nano-objects. Single nanospheres with a diameter of 27 nm are used to map the optical field distribution in the vicinity of the focal point of a microscope objective. The experimentally obtained images are qualitatively compared with a scalar model. The near-field scanning optical microscopy setup is validated using nanospheres with a diameter of 500 nm.The dynamic temporal behavior is examined with the help of time traces. The study is based on the autocorrelation function for single molecules, and using an on-off statistics for single nanocrystals/-rods. In the latter case a power law distribution of the on- and off-time probabilities is observed.
Author: Brian Herman
Publisher: Elsevier
ISBN: 0080571395
Category : Science
Languages : en
Pages : 441
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Book Description
Optical Microscopy: Emerging Methods and Applications covers recent technical advances and new approaches to monitoring and altering cell physiology, examining membrane cytoarchitecture, observing multiple cellular activities and intact organ physiology, plus confocal imaging of live cell function, lifetime imaging, and automated clinical imaging cytometry. The book provides the reader with a synopsis of the most recent technical developments in optical microscopy as applied to scientific research. Each chapter introduces new methods by describing how these overcome limitations inherent in previous techniques. Software, hardware, and other equipment concerns are covered. Additionally, the book reviews current applications in order to stimulate future developments in optical microscopy, encouraging novel uses and new technical advances Caged compounds, fluorescence ratio imaging, and CCD video cameras Simultaneous multiple detection and real-time fluorescence microscopy Simultaneous DIC and quantitative LLF video imaging Total internal reflectance, time-resolved, and automated fluorescence microscopy Laser-scanning confocal microscopy Imaging for calcium measurements, membranes, glycoproteins, living cells, and cancer cells
Author: Tony Wilson
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 456
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Book Description
Numerous applications of confocal microscopes include the life sciences, ophthamology, industrial inspection and semiconductor linewidth metrology. Concentrating on the science and applications of confocal microscopy, this book includes all the latest developments in three-dimensional processing techniques.
