Second Harmonic Generation Imaging

Second Harmonic Generation Imaging PDF Author: Francesco S. Pavone
Publisher: Taylor & Francis
ISBN: 1439849153
Category : Science
Languages : en
Pages : 465

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Book Description
Second-harmonic generation (SHG) microscopy has shown great promise for imaging live cells and tissues, with applications in basic science, medical research, and tissue engineering. Second Harmonic Generation Imaging offers a complete guide to this optical modality, from basic principles, instrumentation, methods, and image analysis to biomedical a

Second Harmonic Generation Imaging

Second Harmonic Generation Imaging PDF Author: Francesco S. Pavone
Publisher: Taylor & Francis
ISBN: 1439849153
Category : Science
Languages : en
Pages : 465

Get Book

Book Description
Second-harmonic generation (SHG) microscopy has shown great promise for imaging live cells and tissues, with applications in basic science, medical research, and tissue engineering. Second Harmonic Generation Imaging offers a complete guide to this optical modality, from basic principles, instrumentation, methods, and image analysis to biomedical a

Second Harmonic Generation Imaging

Second Harmonic Generation Imaging PDF Author: Francesco Pavone
Publisher:
ISBN:
Category : Fluorescence microscopy
Languages : en
Pages : 0

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Book Description
Second-harmonic generation (SHG) microscopy has shown great promise for imaging live cells and tissues, with applications in basic science, medical research, and tissue engineering. Second Harmonic Generation Imaging offers a complete guide to this optical modality, from basic principles, instrumentation, methods, and image analysis to biomedical a.

Optical Imaging and Microscopy

Optical Imaging and Microscopy PDF Author: Peter Török
Publisher: Springer
ISBN: 3540695656
Category : Science
Languages : en
Pages : 504

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Book Description
This text on contemporary optical systems is intended for optical researchers and engineers, graduate students and optical microscopists in the biological and biomedical sciences. In three sections, the book discusses high-aperture optical systems, nonlinear optical techniques, and various techniques that are finding new applications. The new second edition has been thoroughly revised and expanded to account for new advances in fluorescence imaging and diffractive optical lenses.

Quantitative Imaging in Cell Biology

Quantitative Imaging in Cell Biology PDF Author:
Publisher: Academic Press
ISBN: 0124202012
Category : Science
Languages : en
Pages : 588

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Book Description
This new volume, number 123, of Methods in Cell Biology looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications. The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detector impact the quality of quantitative data, and include step-by-step protocols for capturing and analyzing quantitative images. Common quantitative applications, including co-localization, ratiometric imaging, and counting molecules, are covered in detail. Practical chapters cover topics critical to getting the most out of your imaging system, from microscope maintenance to creating standardized samples for measuring resolution. Later chapters cover recent advances in quantitative imaging techniques, including super-resolution and light sheet microscopy. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material

Handbook of Biomedical Nonlinear Optical Microscopy

Handbook of Biomedical Nonlinear Optical Microscopy PDF Author: Barry R. Masters
Publisher: Oxford University Press
ISBN: 9780198036821
Category : Science
Languages : en
Pages : 896

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Book Description
The Handbook of Biomedical Nonlinear Optical Microscopy provides comprehensive treatment of the theories, techniques, and biomedical applications of nonlinear optics and microscopy for cell biologists, life scientists, biomedical engineers, and clinicians. The chapters are separated into basic and advanced sections, and provide both textual and graphical illustrations of all key concepts. The more basic sections are aimed at life scientists without advanced training in physics and mathematics, and tutorials are provided for the more challenging sections. The first part of the Handbook introduces the historical context of nonlinear microscopy. The second part presents the nonlinear optical theory of two- and multiphoton excited fluorescence (TPE, MPE) spectroscopy, second and third harmonic generation (SHG, THG) spectroscopy, and coherent anti-Stokes Raman spectroscopy (CARS). The third part introduces modern microscopic and spectroscopic instrumentation and techniques that are based on nonlinear optics. The fourth part provides key applications of nonlinear microscopy to the biomedical area: neurobiology, immunology, tumor biology, developmental biology, dermatology, and cellular metabolism. There are also chapters on nonlinear molecular probes, cellular damage, and nanoprocessing.

Second-order Nonlinear Optical Characterization Techniques

Second-order Nonlinear Optical Characterization Techniques PDF Author: Thierry Verbiest
Publisher: CRC Press
ISBN: 9781420070736
Category : Technology & Engineering
Languages : en
Pages : 192

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Book Description
Although chemists, biochemists, biologists, and material scientists are often interested in using nonlinear optical techniques for characterizing their samples, they seldom have the necessary background to exploit these methods. Designed for nonspecialists, Second-Order Nonlinear Optical Characterization Techniques: An Introduction focuses on the potential of second-order nonlinear optics as a powerful characterization tool. Avoiding extensive mathematical details, this multidisciplinary book does not require a background in advanced mathematics or physics. After introducing linear optics from the perspective of polarizability and linear susceptibility, the authors cover incoherent second-harmonic generation. They then deal with the study of surfaces and interfaces, exploiting the intrinsic surface sensitivity of second-harmonic generation and sum-frequency generation. The final chapter discusses second-order imaging techniques, including confocal microscopy and two-photon excited fluorescence microscopy. Accessible to a wide range of scientists, this concise book stresses the reliability of nonlinear optical processes for probing surfaces and interfaces. Drawing on the insight offered in the text, scientists from many disciplines can now clearly understand and use second-order nonlinear optical methods.

Applied Nonlinear Optics

Applied Nonlinear Optics PDF Author: Frits Zernike
Publisher: Courier Corporation
ISBN: 048645360X
Category : Science
Languages : en
Pages : 212

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Book Description
Directed toward physicists and engineers interested in the device applications enabled by nonlinear optics, this text is suitable for advanced undergraduates and graduate students. Its content is presented entirely on a classical basis and requires only an elementary knowledge of quantum mechanics. The authors demonstrate how real laboratory situations can diverge from ideal theory, acquainting readers with the kinds of problems common to construction of a nonlinear device. They also offer a detailed discussion of the practical problems and characteristics of nonlinear materials, as well as the selection procedures necessary to ensure the use of good material. Their treatment begins with an introduction to the theories of linear and nonlinear optics, along with the basic ideas behind them. Succeeding chapters explore phase matching and nonlinear materials, followed by detailed treatments of second-harmonic generation, parametric up-conversion, and optical parametric amplification and oscillation. Appendixes offer a comprehensive list of materials and their properties; the text concludes with references and an index.

Toxic Chemical and Biological Agents

Toxic Chemical and Biological Agents PDF Author: Giovanni Sindona
Publisher: Springer Nature
ISBN: 940242041X
Category : Science
Languages : en
Pages : 282

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Book Description
This book critically assesses the current state of knowledge on new and important detection technologies, e.g. mass spectrometry, tandem mass spectrometry, biosensor detection and tissue imaging, in connection with toxic chemical and biological agents. In general, the main topics discussed concern the risks and consequences of chemical and biological agents for human health in general, with special emphasis on all biochemical and metabolic pathways including the reproductive system. The exposome, genetic risks and the environment, various health hazard agents, risk assessment, environmental assessment and preparedness, and analysis of sub-lethal effects at the molecular level are also discussed. In closing, the book provides comprehensive information on the diagnosis of exposure, and on health concerns related to toxic chemical and biological agents.

Nanoscale Photonic Imaging

Nanoscale Photonic Imaging PDF Author: Tim Salditt
Publisher: Springer Nature
ISBN: 3030344134
Category : Science
Languages : en
Pages : 634

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Book Description
This open access book, edited and authored by a team of world-leading researchers, provides a broad overview of advanced photonic methods for nanoscale visualization, as well as describing a range of fascinating in-depth studies. Introductory chapters cover the most relevant physics and basic methods that young researchers need to master in order to work effectively in the field of nanoscale photonic imaging, from physical first principles, to instrumentation, to mathematical foundations of imaging and data analysis. Subsequent chapters demonstrate how these cutting edge methods are applied to a variety of systems, including complex fluids and biomolecular systems, for visualizing their structure and dynamics, in space and on timescales extending over many orders of magnitude down to the femtosecond range. Progress in nanoscale photonic imaging in Göttingen has been the sum total of more than a decade of work by a wide range of scientists and mathematicians across disciplines, working together in a vibrant collaboration of a kind rarely matched. This volume presents the highlights of their research achievements and serves as a record of the unique and remarkable constellation of contributors, as well as looking ahead at the future prospects in this field. It will serve not only as a useful reference for experienced researchers but also as a valuable point of entry for newcomers.

Quantum Imaging

Quantum Imaging PDF Author: Mikhail I. Kolobov
Publisher: Springer Science & Business Media
ISBN: 0387339884
Category : Science
Languages : en
Pages : 321

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Book Description
This book gives an overview of the latest progress in the domain of quantum imaging. It reflects three and a half years of research carried out by leading specialists in the area within the Quantum Imaging network, a research programme of the European Community. Quantum Imaging is a newly born branch of quantum optics that investigates the ultimate performance limits of optical imaging allowed by the laws of quantum mechanics. Using the methods and techniques from quantum optics, quantum imaging addresses the questions of image formation, processing and detection with sensitivity and resolution exceeding the limits of classical imaging.