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Author: Yinong Yang
Publisher: Humana Press
ISBN: 9781627031936
Category : Science
Languages : en
Pages : 0
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Book Description
With the completion of a finished rice genome sequence, increasing efforts have focused on functional characterization of rice genes, elucidation of the underlying mechanisms involved in major agronomic traits (e.g., high yield, grain quality, abiotic stress tolerance, and disease resistance), and the subsequent translation of genomic knowledge into agricultural productivity via molecular breeding and improved cultural practice. To meet increasing interest in this field, Rice Protocols has been compiled to provide a series of core techniques and approaches commonly used in studying rice molecular biology and functional genomics. These approaches include genetic and molecular techniques such as artificial hybridization, fluorescence in situ hybridization, generation and characterization of chemical and T-DNA insertional mutants, quantitative trait loci (QTLs) analysis and map-based cloning, site-specific transgene integration, and artificial microRNA-mediated gene silencing, along with a variety of “omics” techniques. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Rice Protocols will prove useful for both beginners and experienced researchers whether they are molecular biologists who want to study rice plants or rice researchers who are interested in learning molecular techniques.
Author: Yinong Yang
Publisher: Humana Press
ISBN: 9781627031936
Category : Science
Languages : en
Pages : 0
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Book Description
With the completion of a finished rice genome sequence, increasing efforts have focused on functional characterization of rice genes, elucidation of the underlying mechanisms involved in major agronomic traits (e.g., high yield, grain quality, abiotic stress tolerance, and disease resistance), and the subsequent translation of genomic knowledge into agricultural productivity via molecular breeding and improved cultural practice. To meet increasing interest in this field, Rice Protocols has been compiled to provide a series of core techniques and approaches commonly used in studying rice molecular biology and functional genomics. These approaches include genetic and molecular techniques such as artificial hybridization, fluorescence in situ hybridization, generation and characterization of chemical and T-DNA insertional mutants, quantitative trait loci (QTLs) analysis and map-based cloning, site-specific transgene integration, and artificial microRNA-mediated gene silencing, along with a variety of “omics” techniques. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Rice Protocols will prove useful for both beginners and experienced researchers whether they are molecular biologists who want to study rice plants or rice researchers who are interested in learning molecular techniques.
Author: Fatma Sarsu
Publisher: Springer
ISBN: 3319773380
Category : Science
Languages : en
Pages : 39
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Book Description
This open access book presents simple, robust pre-field screening protocols that allow plant breeders to screen for enhanced tolerance to heat stress in rice. Two critical heat-sensitive stages in the lifecycle of the rice crop are targeted – the seedling and flowering stages – with screening based on simple phenotypic responses. The protocols are based on the use of a hydroponics system and/or pot experiments in a glasshouse in combination with a controlled growth chamber where the heat stress treatment is applied. The protocols are designed to be effective, simple, reproducible and user-friendly. The protocols will enable plant breeders to effectively reduce the number of plants from a few thousands to less than 100 candidate individual mutants or lines in a greenhouse/growth chamber, which can then be used for further testing and validation in the field conditions. The methods can also be used to classify rice genotypes according to their heat tolerance characteristics. Thus, different types of heat stress tolerance mechanisms can be identified, presenting opportunities for pyramiding different (mutant) sources of heat stress tolerance.
Author: Nese Sreenivasulu
Publisher: Humana
ISBN: 9781493994106
Category : Science
Languages : en
Pages : 338
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Book Description
This volume explores the latest advances in high-throughput phenotyping to screen milling quality, cooking and nutritional quality in breeding material, and simplifying grain quality screening in germplasm. The chapters in this book cover topics such as seed development stages; panicle architectural traits to understand yield components; starch structure and protein properties; and genome editing technologies to reveal grain quality and nutritional traits. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Rice Grain Quality: Methods and Protocols is a valuable resource for all researchers interested in expanding their knowledge in this evolving field.
Author: Souleymane Bado
Publisher: Springer
ISBN: 3319265903
Category : Science
Languages : en
Pages : 37
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Book Description
This book offers effective, low-cost and user-friendly protocols for the pre-field selection of salt-tolerant mutants in cereal crops. It presents simple methods for measuring soil salinity, including soil sampling and the analysis of water-soluble salts, and describes a detailed, but simple, screening test for salt tolerance in rice, wheat and barley seedlings, which uses hydroponics. The protocols are devised for use by plant breeders and can be easily accommodated into breeding practice.
Author: Souleymane Bado
Publisher: Springer
ISBN: 9783319265889
Category : Science
Languages : en
Pages : 0
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Book Description
This book offers effective, low-cost and user-friendly protocols for the pre-field selection of salt-tolerant mutants in cereal crops. It presents simple methods for measuring soil salinity, including soil sampling and the analysis of water-soluble salts, and describes a detailed, but simple, screening test for salt tolerance in rice, wheat and barley seedlings, which uses hydroponics. The protocols are devised for use by plant breeders and can be easily accommodated into breeding practice.
Author: Gary R. Reinl
Publisher:
ISBN: 9780989831918
Category : Cold
Languages : en
Pages : 165
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Book Description
Author: Yinong Yang
Publisher: Humana Press
ISBN: 9781493957644
Category : Science
Languages : en
Pages : 335
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Book Description
With the completion of a finished rice genome sequence, increasing efforts have focused on functional characterization of rice genes, elucidation of the underlying mechanisms involved in major agronomic traits (e.g., high yield, grain quality, abiotic stress tolerance, and disease resistance), and the subsequent translation of genomic knowledge into agricultural productivity via molecular breeding and improved cultural practice. To meet increasing interest in this field, Rice Protocols has been compiled to provide a series of core techniques and approaches commonly used in studying rice molecular biology and functional genomics. These approaches include genetic and molecular techniques such as artificial hybridization, fluorescence in situ hybridization, generation and characterization of chemical and T-DNA insertional mutants, quantitative trait loci (QTLs) analysis and map-based cloning, site-specific transgene integration, and artificial microRNA-mediated gene silencing, along with a variety of “omics” techniques. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Rice Protocols will prove useful for both beginners and experienced researchers whether they are molecular biologists who want to study rice plants or rice researchers who are interested in learning molecular techniques.
Author: M. Maluszynski
Publisher: Springer Science & Business Media
ISBN: 940171293X
Category : Science
Languages : en
Pages : 451
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Book Description
The production of doubled haploids has become a necessary tool in advanced plant breeding institutes and commercial companies for breeding many crop species. However, the development of new, more efficient and cheaper large scale production protocols has meant that doubled haploids are also recently being applied in less advanced breeding programmes. This Manual was prepared to stimulate the wider use of this technology for speeding and opening up new breeding possibilities for many crops including some woody tree species. Since the construction of genetic maps using molecular markers requires the development of segregating doubled haploid populations in numerous crop species, we hope that this Manual will also help molecular biologists in establishing such mapping populations. For many years, both the Food and Agriculture Organization of the United Nations (FAO) and the International Atomic Energy Agency (IAEA) have supported and coordinated research that focuses on development of more efficient doubled haploid production methods and their applications in breeding of new varieties and basic research through their Plant Breeding and Genetics Section of the Joint F AO/IAEA Division of Nuclear Techniques in Food and Agriculture. The first F AO/IAEA scientific network (Coordinated Research Programme - CRP) dealing with doubled haploids was initiated by the Plant Breeding and Genetics Section in 1986.
Author: Mirza Hasanuzzaman
Publisher: Woodhead Publishing
ISBN: 0128143339
Category : Business & Economics
Languages : en
Pages : 986
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Book Description
Advances in Rice Research for Abiotic Stress Tolerance provides an important guide to recognizing, assessing and addressing the broad range of environmental factors that can inhibit rice yield. As a staple food for nearly half of the world’s population, and in light of projected population growth, improving and increasing rice yield is imperative. This book presents current research on abiotic stresses including extreme temperature variance, drought, hypoxia, salinity, heavy metal, nutrient deficiency and toxicity stresses. Going further, it identifies a variety of approaches to alleviate the damaging effects and improving the stress tolerance of rice. Advances in Rice Research for Abiotic Stress Tolerance provides an important reference for those ensuring optimal yields from this globally important food crop. Covers aspects of abiotic stress, from research, history, practical field problems faced by rice, and the possible remedies to the adverse effects of abiotic stresses Provides practical insights into a wide range of management and crop improvement practices Presents a valuable, single-volume sourcebook for rice scientists dealing with agronomy, physiology, molecular biology and biotechnology
Author: IRRI
Publisher: IRRI
ISBN:
Category : Science
Languages : en
Pages : 72
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Book Description
In this manual, we provide detailed protocols for screening rice under the major stresses affecting both rainfed and irrigated rice: submergence, salinity, drought, and the soil problems – excess iron and aluminum. We also include protocols for a new area of abiotic stress research since it is increasingly occurring in farmers’ field due to climate change: combined stresses. Results from these protocols on combined drought + salinity and submergence + salinity indicate that the effects on the rice plant are sometimes different than under individual stresses. The protocols in this manual have been developed and optimized over decades at IRRI. Numerous scientists, technical staff and students have been involved in the development and use of these protocols, and it is often their experiences that have led to improvements, all of which are highly acknowledged. Our objective with this compilation is to provide a resource for researchers around the globe who aim to improve the abiotic stress tolerance of rice as part of the effort to benefit rice farmers in both rainfed and irrigated areas affected by abiotic stresses
