Principles of Three Dimensional Imaging in Confocal Microscopes

Principles of Three Dimensional Imaging in Confocal Microscopes PDF Author: Min Gu
Publisher: World Scientific
ISBN: 9789810225506
Category : Science
Languages : en
Pages : 360

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Book Description
This book discusses the various principles in confocal scanning microscopy which has become a useful tool in many practical fields including biological studies and industrial inspection. The methodology presented in this book is unique and is based on the concept of the three-dimensional transfer functions which have been developed by the author and his colleagues over the last five years. With the 3-D transfer functions, resolving power in 3-D confocal imaging can be defined in a unified way, different optical arrangements can be compared with an insight into their inter-relationship, and images of thick objects can be modeled in terms of the Fourier transform which makes the analysis easy. The aim of this book is to provide a systematic introduction to the concept of the 3-D transfer functions in various confocal microscopes, to describe the methods for the derivation of different 3-D transfer functions, and to explain the principles of 3-D confocal imaging in terms of these functions.

Principles of Three Dimensional Imaging in Confocal Microscopes

Principles of Three Dimensional Imaging in Confocal Microscopes PDF Author: Min Gu
Publisher: World Scientific
ISBN: 9789810225506
Category : Science
Languages : en
Pages : 360

Get Book

Book Description
This book discusses the various principles in confocal scanning microscopy which has become a useful tool in many practical fields including biological studies and industrial inspection. The methodology presented in this book is unique and is based on the concept of the three-dimensional transfer functions which have been developed by the author and his colleagues over the last five years. With the 3-D transfer functions, resolving power in 3-D confocal imaging can be defined in a unified way, different optical arrangements can be compared with an insight into their inter-relationship, and images of thick objects can be modeled in terms of the Fourier transform which makes the analysis easy. The aim of this book is to provide a systematic introduction to the concept of the 3-D transfer functions in various confocal microscopes, to describe the methods for the derivation of different 3-D transfer functions, and to explain the principles of 3-D confocal imaging in terms of these functions.

Three-Dimensional Confocal Microscopy: Volume Investigation of Biological Specimens

Three-Dimensional Confocal Microscopy: Volume Investigation of Biological Specimens PDF Author:
Publisher: Academic Press
ISBN: 0323138969
Category : Science
Languages : en
Pages : 507

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Book Description
The integration of confocal microscopy and volume investigation has led to an unprecedented ability to examine spatial relationships between cellular structure and function. The goal of this book is to familiarize the reader with these new technologies and to demonstrate their applicability to a wide range of biological and clinical problems. Volume investigation Three-dimensional reconstruction Fluroescent probe design Biological applications of confocal microscopy, including calcium imaging, receptor movement, and diagnostic pathology Confocal data display and analysis Twenty-eight pages of color

Electron Tomography

Electron Tomography PDF Author: Joachim Frank
Publisher: Springer Science & Business Media
ISBN: 1475721633
Category : Science
Languages : en
Pages : 398

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Book Description
This unique resource details the theory, working methods, and applications of electron tomographic techniques for imaging asymmetric, noncrystalline biological specimens.

Three-dimensional Microscopy

Three-dimensional Microscopy PDF Author:
Publisher:
ISBN:
Category : Confocal microscopy
Languages : en
Pages : 320

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Book Description


Multi-modality Microscopy

Multi-modality Microscopy PDF Author: Hanry Yu
Publisher: World Scientific
ISBN: 9814479128
Category : Technology & Engineering
Languages : en
Pages : 317

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Book Description
This book covers important aspects of modern optical microscopy and image restoration technologies. Instead of pure optical treatment, the book is delivered with the consideration of the scientists who utilize optical microscopy in their daily research. However, enough details are provided in basic imaging principles, optics and instrumentation in microscopy, spherical aberrations, deconvolution and image restoration. A number of microscopic technologies such as polarization, confocal and multi-photon microscopy are highlighted with their applications in biological and materials sciences/engineering.

Three-Dimensional Microscopy

Three-Dimensional Microscopy PDF Author:
Publisher:
ISBN:
Category :
Languages : en
Pages :

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Book Description


Confocal Microscopy and Multiphoton Excitation Microscopy

Confocal Microscopy and Multiphoton Excitation Microscopy PDF Author: Barry R. Masters
Publisher: SPIE Press
ISBN: 9780819461186
Category : Confocal microscopy
Languages : en
Pages : 236

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Book Description
This text guides you through the principles and practical techniques of confocal and multiphoton microscopy. It also describes the historical connections and parallel inventions that resulted in modern techniques of live cell imaging and their use in biology and medicine. You will find comparisons of different types of confocal and multiphoton microscopes, solutions to the problems one would encounter when using various microscopic techniques, tips on selecting equipment, and an extensive annotated bibliography of additional resources.

Confocal Microscopy

Confocal Microscopy PDF Author: Jian Liu
Publisher: Morgan & Claypool Publishers
ISBN: 1681743388
Category : Science
Languages : en
Pages : 108

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Book Description
The confocal microscope is appropriate for imaging cells or the measurement of industrial artefacts. However, junior researchers and instrument users sometimes misuse imaging concepts and metrological characteristics, such as position resolution in industrial metrology and scale resolution in bio-imaging. And, metrological characteristics or influence factors in 3D measurement such as height assessment error caused by 3D coupling effect are so far not yet identified. In this book, the authors outline their practices by the working experiences on standardization and system design. This book assumes little previous knowledge of optics, but rich experience in engineering of industrial measurements, in particular with profile metrology or areal surface topography will be very helpful to understand the theoretical concerns and value of the technological advances. It should be useful for graduate students or researchers as extended reading material, as well as microscope users alongside their handbook.

Proceedings of Three-dimensional Microscopy

Proceedings of Three-dimensional Microscopy PDF Author:
Publisher:
ISBN:
Category : Confocal microscopy
Languages : en
Pages : 296

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Book Description


Confocal Microscopy

Confocal Microscopy PDF Author: Tony Wilson
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 456

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Book Description
Numerous applications of confocal microscopes include the life sciences, ophthamology, industrial inspection and semiconductor linewidth metrology. Concentrating on the science and applications of confocal microscopy, this book includes all the latest developments in three-dimensional processing techniques.