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Author: Per-Ake Albertsson
Publisher: Wiley-Interscience
ISBN: 9780471828204
Category : Science
Languages : en
Pages : 346
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Book Description
Describes partition techniques for the separation and purification of cells, cell organelles, membrane vesicles, viruses, and biopolymers, such as proteins and nucleic acids. The basic theory of partition is discussed, as are the properties of aqueous, two-phase systems and the general behavior of particles and molecules. Updates include advances and new applications introduced since 1971.
Author: Per-Ake Albertsson
Publisher: Wiley-Interscience
ISBN: 9780471828204
Category : Science
Languages : en
Pages : 346
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Book Description
Describes partition techniques for the separation and purification of cells, cell organelles, membrane vesicles, viruses, and biopolymers, such as proteins and nucleic acids. The basic theory of partition is discussed, as are the properties of aqueous, two-phase systems and the general behavior of particles and molecules. Updates include advances and new applications introduced since 1971.
Author: Per Åke Albertsson
Publisher:
ISBN:
Category : Biochemistry
Languages : en
Pages : 248
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Book Description
Author: Harry Walter
Publisher: Elsevier
ISBN: 9780121821296
Category : Nature
Languages : en
Pages : 768
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Book Description
General methodology and apparatus: phase diagrams, preparation and analysis of two-phase systems, partioning and affinity partitioning of macromolecules: Proteins, nucleic acids, studies on protein interactionsmolecular structure, charge, hydrophobicity, and conformational chan ges, partitioning and affinity partitioning of particulates,organellesseparation and subfractionation, menbrane: separation and subfractionation, membrane domain analysis, aqueous phase separation in biologicalsystems, aqueous two-phase systems in large-scale process biotechnolo gy, proteins; downstream processing, design of proteins for enhanced extraction, other applications of aqueous phases in biotechnology. Enzymology.
Author: Munishwar N. Gupta
Publisher: Springer Science & Business Media
ISBN: 9783764363055
Category : Science
Languages : en
Pages : 240
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Book Description
One major concern of biotechnology is either using enzymes or producing them. Enzyme/protein production is therefore an important starting point for biotechnology. Bioseparation or Downstream Processing constitutes about 40-90% of the total production cost. Driven by economics, highly selective technologies applicable to large-scale processing have emerged during the last decade. These technologies are slowly diffusing to enzymologists who are working on a smaller scale, looking for fast and efficient purification protocols. The affinity-based techniques (including precipitation, two-phase extractions, expanded bed chromatography, perfusion chromatography and monoliths) described in this volume provide current and new cutting-edge methods. Consequently, the book is of main interest to researchers in biochemistry, biochemical engineering and biotechnology, working either in academic or industrial sectors.
Author: Per Åke Albertsson
Publisher:
ISBN:
Category : Cell fractionation
Languages : en
Pages : 346
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Book Description
Author: Robert K. Scopes
Publisher: Springer Science & Business Media
ISBN: 9780387940724
Category : Science
Languages : en
Pages : 408
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Book Description
New textbooks at all levels of chemistry appear with great regularity. Some fields such as basic biochemistry, organic re action mechanisms, and chemical thermodynamics are weil represented by many excellent texts, and new or revised editions are published sufficiently often to keep up with progress in research. However, some areas of chemistry, especially many of those taught at the graduate level, suffer from a real lack of up-to-date textbooks. The most serious needs occur in fields that are rapidly changing. Textbooks in these subjects usually have to be written by scientists actually involved in the research that is advancing the field. It is not often easy to persuade such individuals to set time aside to help spread the knowledge they have accumulated. Our goal, in this series, is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks, and then seek out and persuade experts in these fields to produce relatively concise but instruc tive introductions to their fields. These should serve the needs of one semester or one quarter graduate courses in chemistry and biochemistry. In so me cases the availability of texts in active research areas should help stimulate the creation of new courses.
Author: D. Fisher
Publisher: Springer Science & Business Media
ISBN: 1468456679
Category : Science
Languages : en
Pages : 476
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Book Description
The use of aqueous two-phase systems for the partitioning of macromolecules, organelles and cells was originally developed by Per-Ake Albertsson in Sweden in the mid-fifties [1-3]. These systems were initially applied to separations of plant organelles and viruses but their use has now extended into most areas of cell biology and biochemistry [4,5]. Since 1979 biennial International Conferences on Partitioning in Aqueous Two-Phase Systems have been held in Los Angeles (1979), Sheffield (1981), Vancouver (1983) and Lund (1985). The 5th conference was held in Oxford from 23-28 August 1987 and was entitled "Advances in Separations Using Aqueous Phase Systems in Cell Biology and Biotechnology". It is the formal presentations from this meeting which comprise this volume. In contrast to earlier books on phase partitioning [4,5] this volume contains, for the first time, worldwide contributions from over sixty partitioners from a variety of scientific disciplines, thereby providing a detailed overview of the widespread application and potential of bioseparations using phase partitioning. Disciplines include Biophysics, Biochemistry, Cell Biology, Microbiology, Biotechnology and Process Engineering, in both academic and commercial establishments. These biennial conferences allow advances in these diverse partitioning fields to be reviewed and compared; they also provide an opportunity for those considering using phase partitioning to obtain information, advice and contacts. Attendance has grown steadily over the years and 140 scientists came to Oxford. The conference consisted of ten symposia on areas of application of partitioning which have been organised as specific chapters in this volume.
Author: Harry Walter
Publisher: Elsevier
ISBN: 0323142915
Category : Science
Languages : en
Pages : 730
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Book Description
Partitioning in Aqueous Two-Phase Systems: Theory, Methods, Uses, and Applications to Biotechnology is a collection of papers that discusses the applications of aqueous two-phase systems to problems of separation and extraction of macromolecules, organelles, and cells. Papers focus on the theoretical basis and the practical details of the procedures used. Some of the papers describe in one or a few steps how two components can be separated by the investigator manipulating their partitions so that one component is in one phase and the other component is in the other phase or at the interface. Investigators can also avail of developed batch extractions for plant organelles, cell membranes, nucleic acids, and proteins. The book cites as an example the partitioning of right-side-out and inside-out vesicles (obtained from fragments of thylakoid membranes) to the top and bottom phases, respectively, of a Dx-PEG system. Other papers describe the use of the countercurrent distribution when single extraction steps are not sufficient to produce a separation in materials that do not differ greatly in their partitioning behavior. The collection can prove valuable for bio-chemists, cellular biologists, micro-biologists, and developmental biologists.
Author: Bruce Alberts
Publisher:
ISBN: 9780815332183
Category : Cytology
Languages : en
Pages : 0
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Book Description
Author: Robin D. Rogers
Publisher: Springer Science & Business Media
ISBN: 1461519535
Category : Science
Languages : en
Pages : 191
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Book Description
Proceedings of an American Chemical Society Symposium held in San Diego, California, March 13-14, 1994
