Multidimensional Microscopy

Multidimensional Microscopy PDF Author: P.-C. Cheng
Publisher: Springer Science & Business Media
ISBN: 1461383668
Category : Science
Languages : en
Pages : 394

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Book Description
Modern cell biology is being revolutionized by the wedding of microscopy and computers. This book describes the new instrumentation and methods which allow three-dimensional reconstruction of specimens. Multidimensional Microscopy will be of interest to cell biologists, microscopists, and basic biomedical researchers whose work involves microscopic techniques. This book presents current results on a very active field in modern biology: methods in light and electron microscopy that allow the reconstruction of three-dimensional objects with the aid of computers. The book emphasizes the methods that can be used and examples of biological systems to which they have been applied. It includes extensive descriptions of confocal microscopy and its applications, as well as chapters on X-ray microscopy, low-voltage electron microscopy, and image reconstruction. This is an impressive summary of state-of-the-art methods in microscopy, in which microscopes and computers are being joined to permit specimens to be examined and reconstructed in three dimensions. Will be of interest to cell biologists, biomedical researchers, and microscopists.

Multidimensional Microscopy

Multidimensional Microscopy PDF Author: P.-C. Cheng
Publisher: Springer Science & Business Media
ISBN: 1461383668
Category : Science
Languages : en
Pages : 394

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Book Description
Modern cell biology is being revolutionized by the wedding of microscopy and computers. This book describes the new instrumentation and methods which allow three-dimensional reconstruction of specimens. Multidimensional Microscopy will be of interest to cell biologists, microscopists, and basic biomedical researchers whose work involves microscopic techniques. This book presents current results on a very active field in modern biology: methods in light and electron microscopy that allow the reconstruction of three-dimensional objects with the aid of computers. The book emphasizes the methods that can be used and examples of biological systems to which they have been applied. It includes extensive descriptions of confocal microscopy and its applications, as well as chapters on X-ray microscopy, low-voltage electron microscopy, and image reconstruction. This is an impressive summary of state-of-the-art methods in microscopy, in which microscopes and computers are being joined to permit specimens to be examined and reconstructed in three dimensions. Will be of interest to cell biologists, biomedical researchers, and microscopists.

Focus on Multidimensional Microscopy

Focus on Multidimensional Microscopy PDF Author: Ping-chin Cheng
Publisher: World Scientific
ISBN: 9789810230531
Category : Confocal microscopy
Languages : en
Pages : 326

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Book Description
This book covers various aspects of modern microscopy, with emphasis on multidimensional (three-dimensional and higher) and multimodality microscopy. The topics discussed include multiphoton fluorescent microscopy, confocal microscopy, x-ray microscopy and microtomography, electron microscopy, probe microscopy and multidimensional image processing for microscopy. In addition, there are chapters demonstrating typical microscopical applications, both biological and material

Focus On Multidimensional Microscopy (In 2 Vols) - Volume 1

Focus On Multidimensional Microscopy (In 2 Vols) - Volume 1 PDF Author: Ping-chin Cheng
Publisher: World Scientific
ISBN: 9814497711
Category : Science
Languages : en
Pages : 320

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Book Description
This book covers various aspects of modern microscopy, with emphasis on multidimensional (three-dimensional and higher) and multimodality microscopy. The topics discussed include multiphoton fluorescent microscopy, confocal microscopy, x-ray microscopy and microtomography, electron microscopy, probe microscopy and multidimensional image processing for microscopy. In addition, there are chapters demonstrating typical microscopical applications, both biological and material.

Focus on Multidimensional Microscopy

Focus on Multidimensional Microscopy PDF Author: P C Cheng
Publisher: World Scientific
ISBN: 981449772X
Category : Science
Languages : en
Pages : 660

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Book Description
This book covers various aspects of modern microscopy, with emphasis on multidimensional (three-dimensional and higher) and multimodality microscopy. The topics discussed include multiphoton fluorescent microscopy, confocal microscopy, x-ray microscopy and microtomography, electron microscopy, probe microscopy and multidimensional image processing for microscopy. In addition, there are chapters demonstrating typical microscopical applications, both biological and material. Contents: Fluorescence Lifetime Imaging by Double Pulse Excitation in Bilateral Confocal Microscopy (G J Brakenhoff et al.)Icosahedral Virus Structure Determination by Electron Cryomicroscopy: Image Processing Principles (P A Thuman-Commike & W Chiu)Appropriate Image Processing for Confocal Microscopy (G Cox & C J R Sheppard)X-Ray Fluorescence Two-Dimensional Microanalysis at the VEPP-3 Storage Ring: Applications in Environmental Science (I P Dolbnya & K V Zolotarev)3D Transfer-Function Analysis for 4Pi Confocal Microscopy (M Gu & C J R Sheppard)Image Restoration of Thick Biological Specimens for Transmission Electron Microscope Tomography (K F Han et al.)Images of Membrane-Bound Detoxification Enzyme at 4Å Resolution Obtained by Electron Cryomicroscopy (I Schmidt-Krey et al.)The Uptake Pathway of DNA and Lipids in Cationic Liposome-Mediated Gene Transfer (S-W Hui et al.)Multiscale Imaging, Analysis and Modeling of the Pulmonary Bronchial Tree (A Kriete et al.)Asymmetric Cell Division as a Mechanism of Cell Determination in Vascular Plants (B-L Lin)3D Electron Microscopy of Cells and Organelles by High-Voltage EM, Tomography and Stereopair Analysis (M Marko)Surface Scattering Techniques in Scanning Optical Microscopy (J F Aguilar & E R Méndez)Applications of Electron Microscopy to Materials and Earth Sciences – Dislocation, Shape and Orientation Changes and Interdiffusion (P Shen)Surface Profiling and Confocal Microscopy (C J R Sheppard & J C Quartel)Matched Spatial Filters in Long Working Distance Microscopy of Phase Objects (J R Strickler & J-S Hwang)Transport Pathways of Lipoproteins Across the Arterial Endothelial Cells (C-H Kao et al.)Polarisation Effects in Confocal Microscopy (T Wilson)Near-Field Microscopy and Spectroscopy of Single Molecules, Single Proteins and Biological Membranes (X S Xie et al.) Keywords:Microscopy;Optics;Image Processing

Focus on Multidimensional Microscopy

Focus on Multidimensional Microscopy PDF Author: Ping-chin Cheng
Publisher:
ISBN: 9789812817112
Category : Science
Languages : en
Pages : 0

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Book Description
1. Fluorescence lifetime imaging by double pulse excitation in bilateral confocal microscopy / G.J. Brakenhoff [und weitere] -- 2. Icosahedral virus structure determination by electron cryomicroscopy: Image processing principles / P.A. Thuman-Commike and W. Chiu -- 3. Appropriate image processing for confocal microscopy / G. Cox and C.J.R. Sheppard -- 4. X-ray fluorescence two-dimensional microanalysis at the VEPP-3 storage ring: Applications in environmental science / I.P. Dolbnya and K.V. Zolotarev -- 5. 3D transfer-function analysis for 4Pi confocal microscopy / M. Gu and C.J.R. Sheppard -- 6. Image restoration of thick biological specimens for transmission electron microscope tomography / K.F. Han [und weitere] -- 7. Images of membrane-bound detoxification enzyme at 4A resolution obtained by electron cryomicroscopy / I. Schmidt-Krey [und weitere] -- 8. The uptake pathway of DNA and lipids in cationic liposome-mediated gene transfer / S.-W. Hui [und weitere] -- 9. Multiscale imaging, analysis and modeling of the pulmonary bronchial tree / A. Kriete [und weitere] -- 10. Asymmetric cell division as a mechanism of cell determination in vascular plants / B.-L. Lin -- 11. 3D electron microscopy of cells and organelles by high-voltage EM, tomography and stereopair analysis / M. Marko -- 12. Surface scattering techniques in scanning optical microscopy / J.F. Aguilar and E.R. Mendez -- 13. Applications of electron microscopy to materials and earth sciences - Dislocation, shape and orientation changes and interdiffusion / P. Shen -- 14. Surface profiling and confocal microscopy / C.J.R. Sheppard and J.C. Quartel -- 15. Matched spatial filters in long working distance microscopy of phase objects / J.R. Strickler and J.-S. Hwang -- 16. Transport pathways of lipoproteins across the arterial endothelial cells / C.-H. Kao [und weitere] -- 17. Polarisation effects in confocal microscopy / T. Wilson -- 18. Near-field microscopy and spectroscopy of single molecules, single proteins and biological membranes / X.S. Xie [und weitere]

Nanoscopy and Multidimensional Optical Fluorescence Microscopy

Nanoscopy and Multidimensional Optical Fluorescence Microscopy PDF Author: Alberto Diaspro
Publisher: CRC Press
ISBN: 1420078895
Category : Technology & Engineering
Languages : en
Pages : 448

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Book Description
"Alberto Diaspro has been choreographing light’s dance for over 20 years, and in Nanoscopy and Multidimensional Optical Fluorescence Microscopy, he has assembled a diverse group of experts to explain the methods they use to coax light to reveal biology’s secrets." — From the Foreword by Daniel Evanko, editor, Nature Methods Nanoscopy and Multidimensional Optical Fluorescence Microscopy demonstrates that the boundaries between sciences do blur at the bottom, especially those that might separate the optical work of physicists and the cellular work of microbiologists. In 18 chapters written by pioneering researchers, this work offers the first comprehensive and current documentation of the cutting-edge research being accomplished in a wide range of photonic devices with revolutionary application. The highlight of the book is its coverage of optical nanoscopy and super-resolution microscopy. The rapid advances in this area over the past few years offer researchers in both photonics and molecular biologya wealth of accomplishment upon which they can build. Offering a complete treatment of this emerging field, this volume: Describes how scientists have exploited the properties of light and its fluorophore partners to overcome the resolution limit of conventional light microscopy Delves into recent ways to minimize the photobleaching that has long hampered many methods including those that have the potential to capture previously unobtainable information on the movements of single molecules Discusses the principles, benefits, and implementation of fluorescence correlation spectroscopy and related methods, which simplifies analysis by limiting light to stationary focal points in a sample Considers the most basic as well as emerging methods for improving three-dimensional optical sectioning microscopy Reviews the basics of FRET (fluorescence resonance energy transfer) and considers its new use for investigating protein complexes The text also introduces those emerging nonfluorescence microscopy methods that can actually exert mechanical forces to trap and move a variety of objects ranging from beads to living cells and cellular organelles. Combining this technique with fluorescence microscopy provides an unparalleled ability to manipulate and visualize biological samples. In the half-century since Richard Feynman challenged scientists to come up with the tools to investigate and manipulate our world at the nanoscale, we have succeeded in placing tools in the hands of biophysicists that are leading to major breakthroughs in our understanding of life and our ability to diagnose, treat, and prevent many challenges to human health. This book reflects what has been accomplished to date while pointing the way to what still needs to be done.

Multi-modality Microscopy

Multi-modality Microscopy PDF Author: Hanry Yu
Publisher: World Scientific
ISBN: 9814479128
Category : Technology & Engineering
Languages : en
Pages : 317

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Book Description
This book covers important aspects of modern optical microscopy and image restoration technologies. Instead of pure optical treatment, the book is delivered with the consideration of the scientists who utilize optical microscopy in their daily research. However, enough details are provided in basic imaging principles, optics and instrumentation in microscopy, spherical aberrations, deconvolution and image restoration. A number of microscopic technologies such as polarization, confocal and multi-photon microscopy are highlighted with their applications in biological and materials sciences/engineering.

Multi-dimensional Imaging

Multi-dimensional Imaging PDF Author: Bahram Javidi
Publisher: John Wiley & Sons
ISBN: 1118706196
Category : Technology & Engineering
Languages : en
Pages : 464

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Book Description
Provides a broad overview of advanced multidimensional imaging systems with contributions from leading researchers in the field Multi-dimensional Imaging takes the reader from the introductory concepts through to the latest applications of these techniques. Split into 3 parts covering 3D image capture, processing, visualization and display, using 1) a Multi-View Approach and 2.) a Holographic Approach, followed by a 3rd part addressing other 3D systems approaches, applications and signal processing for advanced 3D imaging. This book describes recent developments, as well as the prospects and challenges in advances in imaging sciences and engineering such as 3D image sensing, 3D holographic imaging, imaging applications for bio-photonics and 3D image recognition. Advanced imaging systems incorporate knowledge from various fields. It is a complex technology that combines physics, optics, signal processing, and image capture techniques. Provides a broad overview of advanced multidimensional imaging systems with contributions from leading researchers in the field. Integrates the background, introductory material with new advances in 3D imaging and applications. Covers the most recent technologies such as high speed digital holography, compressive sensing, real-time 3D integral imaging, 3D TV, photon counting imaging. To be available as an enhanced ebook with added functionality of colour films showing the effects of advanced 3D applications such as 3D microscopy, 3D biomedical imaging and 3D for security and defense applications. Acts as a single source reference to the rapidly developing field of 3D imaging technology. Provides supplementary material on a companion website including video clips, examples, numerical simulations, and experimental results to show the theoretical concepts. With contributions from leading researchers from across these fields, Multi-dimensional Imaging is a comprehensive reference for the imaging technology research community.

Three-Dimensional and Multidimensional Microscopy

Three-Dimensional and Multidimensional Microscopy PDF Author: Thomas Gordon Brown
Publisher:
ISBN: 9781628419474
Category : Confocal microscopy
Languages : en
Pages : 282

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Book Description
'Proceedings of SPIE' presents the original research papers presented at SPIE conferences and other high-quality conferences in the broad-ranging fields of optics and photonics. These books provide prompt access to the latest innovations in research and technology in their respective fields.

Three-dimensional and Multidimensional Microscopy

Three-dimensional and Multidimensional Microscopy PDF Author:
Publisher:
ISBN:
Category : Confocal microscopy
Languages : en
Pages : 262

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Book Description