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Author: Vasily Astratov
Publisher: Springer Nature
ISBN: 3030217221
Category : Science
Languages : en
Pages : 487
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Book Description
This book presents the advances in super-resolution microscopy in physics and biomedical optics for nanoscale imaging. In the last decade, super-resolved fluorescence imaging has opened new horizons in improving the resolution of optical microscopes far beyond the classical diffraction limit, leading to the Nobel Prize in Chemistry in 2014. This book represents the first comprehensive review of a different type of super-resolved microscopy, which does not rely on using fluorescent markers. Such label-free super-resolution microscopy enables potentially even broader applications in life sciences and nanoscale imaging, but is much more challenging and it is based on different physical concepts and approaches. A unique feature of this book is that it combines insights into mechanisms of label-free super-resolution with a vast range of applications from fast imaging of living cells to inorganic nanostructures. This book can be used by researchers in biological and medical physics. Due to its logically organizational structure, it can be also used as a teaching tool in graduate and upper-division undergraduate-level courses devoted to super-resolved microscopy, nanoscale imaging, microscopy instrumentation, and biomedical imaging.
Author: Vasily Astratov
Publisher: Springer Nature
ISBN: 3030217221
Category : Science
Languages : en
Pages : 487
Get Book
Book Description
This book presents the advances in super-resolution microscopy in physics and biomedical optics for nanoscale imaging. In the last decade, super-resolved fluorescence imaging has opened new horizons in improving the resolution of optical microscopes far beyond the classical diffraction limit, leading to the Nobel Prize in Chemistry in 2014. This book represents the first comprehensive review of a different type of super-resolved microscopy, which does not rely on using fluorescent markers. Such label-free super-resolution microscopy enables potentially even broader applications in life sciences and nanoscale imaging, but is much more challenging and it is based on different physical concepts and approaches. A unique feature of this book is that it combines insights into mechanisms of label-free super-resolution with a vast range of applications from fast imaging of living cells to inorganic nanostructures. This book can be used by researchers in biological and medical physics. Due to its logically organizational structure, it can be also used as a teaching tool in graduate and upper-division undergraduate-level courses devoted to super-resolved microscopy, nanoscale imaging, microscopy instrumentation, and biomedical imaging.
Author: Ji-Xin Cheng
Publisher: Elsevier
ISBN: 0323903371
Category : Science
Languages : en
Pages : 612
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Book Description
Stimulated Raman Scattering Microscopy: Techniques and Applications describes innovations in instrumentation, data science, chemical probe development, and various applications enabled by a state-of-the-art stimulated Raman scattering (SRS) microscope. Beginning by introducing the history of SRS, this book is composed of seven parts in depth including instrumentation strategies that have pushed the physical limits of SRS microscopy, vibrational probes (which increased the SRS imaging functionality), data science methods, and recent efforts in miniaturization. This rapidly growing field needs a comprehensive resource that brings together the current knowledge on the topic, and this book does just that. Researchers who need to know the requirements for all aspects of the instrumentation as well as the requirements of different imaging applications (such as different types of biological tissue) will benefit enormously from the examples of successful demonstrations of SRS imaging in the book. Led by Editor-in-Chief Ji-Xin Cheng, a pioneer in coherent Raman scattering microscopy, the editorial team has brought together various experts on each aspect of SRS imaging from around the world to provide an authoritative guide to this increasingly important imaging technique. This book is a comprehensive reference for researchers, faculty, postdoctoral researchers, and engineers. Includes every aspect from theoretic reviews of SRS spectroscopy to innovations in instrumentation and current applications of SRS microscopy Provides copious visual elements that illustrate key information, such as SRS images of various biological samples and instrument diagrams and schematics Edited by leading experts of SRS microscopy, with each chapter written by experts in their given topics
Author: Udo J. Birk
Publisher: John Wiley & Sons
ISBN: 3527802061
Category : Science
Languages : en
Pages : 260
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Book Description
This unique book on super-resolution microscopy techniques presents comparative, in-depth analyses of the strengths and weaknesses of the individual approaches. It was written for non-experts who need to understand the principles of super-resolution or who wish to use recently commercialized instruments as well as for professionals who plan to realize novel microscopic devices. Explaining the practical requirements in terms of hardware, software and sample preparation, the book offers a wealth of hands-on tips and practical tricks to get a setup running, provides invaluable help and support for successful data acquisition and specific advice in the context of data analysis and visualization. Furthermore, it addresses a wide array of transdisciplinary fields of applications. The author begins by outlining the joint efforts that have led to achieving super-resolution microscopy combining advances in single-molecule photo-physics, fluorophore design and fluorescent labeling, instrument design and software development. The following chapters depict and compare current main standard techniques such as structured illumination microscopy, single-molecule localization, stimulated emission depletion microscopy and multi-scale imaging including light-sheet and expansion microscopy. For each individual approach the experimental setups are introduced, the imaging protocols are provided and the various applications illustrated. The book concludes with a discussion of future challenges addressing issues of routine applications and further commercialization of the available methods. Guiding users in how to make choices for the design of their own experiments from scratch to promising application, this one-stop resource is intended for researchers in the applied sciences, from chemistry to biology and medicine to physics and engineering.
Author: Sebastian Strauß
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Author: Jiang Wu
Publisher: John Wiley & Sons
ISBN: 3527835199
Category : Technology & Engineering
Languages : en
Pages : 293
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Book Description
Optical Imaging and Sensing Understand the future of optical imaging with this cutting-edge guide Optoelectronic devices for imaging and sensing are among the backbones of modern technology. Facilitating the mutual conversion of optical and electrical signals, they have applications from telecommunications to molecular spectroscopy, and their incorporation into photon-involved technologies is only growing. The rapid development of this field makes the need for a fully up-to-date introduction all the more critical. Optical Imaging and Sensing meets this need with a comprehensive guide to the novel materials and devices employed in optical imaging and sensing. Given the current revolution in new imaging materials, an introduction that fully incorporates the latest research is an indispensable tool for scientists and engineers in a huge range of fields. The technologies surveyed here promise to transform public security, 5G and next-generation wireless communication, clinical imaging, and many more. Optical Imaging and Sensing Readers will also find: Detailed discussion of materials including semimetallic graphene, semiconducting black phosphorous, and many more Discussion of devices from infrared photodetectors to nonlinear interferometers A thorough look forward to the future of the field Optical Imaging and Sensing is a useful reference for materials scientists, spectroscopists, semiconductor physicists, and engineers working in any field or industry involving optical imaging or sensing technology.
Author: Aleksei Anatoliyovych Stepanenko
Publisher: Delve Publishing
ISBN: 9781773610320
Category :
Languages : en
Pages : 0
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Book Description
The fluorescence microscope is the principal tool for investigating the spatial organization of bio-molecular processes. Instrumentation and techniques have evolved considerably since the first realizations of fluorescence microscopy. The technology is constantly being advanced to image faster, deeper, less invasively, and more quantitatively, driving discovery of both biological and clinical mechanisms. In this book, we discuss the various approaches, which can be successfully used in live cells, the tradeoffs in resolution, speed, and ease of implementation, which one must make for each approach, and the quality of results that one might expect from each technique. We introduce the recent development of optical microscopy that has achieved spatial resolution beyond the diffraction limit. Super-resolution imaging techniques outperforming the classical diffraction limits are photoactivated localization microscopy (PALM), stochastic optical resolution microscopy (STORM), stimulated-emission depletion microscopy (STED), three-dimensional structured illumation microscopy (3D-SIM) and total internal reflection fluorescence microscopy (TIRF). These super-resolution microscopy techniques have permitted detailed studies of organelles, vesicles, microtubules, DNAs, proteins, and other biochemical particles on the nanometer scale. Super-resolution microscopy has opened the door to true nanoscale mapping of biological components.
Author: Joachim Wegener
Publisher: Springer Nature
ISBN: 3030324338
Category : Science
Languages : en
Pages : 286
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Book Description
This book is dedicated to label-free, non-invasive monitoring of cell-based assays and it comprises the most widely applied techniques. Each approach is described and critically evaluated by an expert in the field such that researchers get an overview on what is possible and where the limitations are. The book provides the theoretical basis for each technique as well as the most successful and exciting applications. Label-free bioanalytical techniques have been known for a long time as valuable tools to monitor adsorption processes at the solid-liquid interface in general – and biomolecular interaction analysis (BIA) in particular. The underlying concepts have been progressively transferred to the analysis of cell-based assays. The strength of these approaches is implicitly given with the name 'label-free': the readout is independent of any label, reagent or additive that contaminates the system under study and potentially affects its properties. Thus, label-free techniques provide an unbiased analytical perspective in the sense that the sample is not manipulated by additives but pure. They are commonly based on physical principles and read changes in integral physical properties of the sample like refractive index, conductivity, capacitance or elastic modulus to mention just a few. Even though it is not implied in the name, label-free approaches usually monitor the cells under study non-invasively meaning that the amplitude of the signal (e.g. electric field strength, mechanical elongation) that is used for the measurement is too low to interfere or affect. In contrast to label-based analytical techniques that are commonly restricted to a single reading at a predefined time point, label-free approaches allow for a continuous observation so that the dynamics of the biological system or reaction become accessible.
Author: Sune Svanberg
Publisher: Springer Nature
ISBN: 3031047761
Category : Science
Languages : en
Pages : 698
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Book Description
A wide-ranging review of modern spectroscopic techniques such as X-ray, photoelectron, optical and laser spectroscopy, and radiofrequency and microwave techniques. On the fundamental side the book focuses on physical principles and the impact of spectroscopy on our understanding of the building blocks of matter, while in the area of applications particular attention is given to those in chemical analysis, photochemistry, surface characterisation, environmental and medical diagnostics, remote sensing and astrophyscis. The Fourth Edition also provides the reader with an update on laser cooling and trapping, Bose-Einstein condensation, ultra-fast spectroscopy, high-power laser/matter interaction, satellite-based astronomy and spectroscopic aspects of laser medicine.
Author: Tommaso Baldacchini
Publisher: William Andrew
ISBN: 0128178280
Category : Technology & Engineering
Languages : en
Pages : 766
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Book Description
Three-Dimensional Microfabrication Using Two-Photon Polymerization, Second Edition offers a comprehensive guide to TPP microfabrication and a unified description of TPP microfabrication across disciplines. It offers in-depth discussion and analysis of all aspects of TPP, including the necessary background, pros and cons of TPP microfabrication, material selection, equipment, processes and characterization. Current and future applications are covered, along with case studies that illustrate the book's concepts. This new edition includes updated chapters on metrology, synthesis and the characterization of photoinitiators used in TPP, negative- and positive-tone photoresists, and nonlinear optical characterization of polymers. This is an important resource that will be useful for scientists involved in microfabrication, generation of micro- and nano-patterns and micromachining. Discusses the major types of nanomaterials used in the agriculture and forestry sectors, exploring how their properties make them effective for specific applications Explores the design, fabrication, characterization and applications of nanomaterials for new Agri-products Offers an overview of regulatory aspects regarding the use of nanomaterials for agriculture and forestry
Author: Ting-Chung Poon
Publisher: MDPI
ISBN: 3039435957
Category : Technology & Engineering
Languages : en
Pages : 364
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Book Description
Modern holographic techniques have been successfully applied in many important areas, such as 3-D inspection, 3-D microscopy, metrology, and profilometry, augmented reality, and industrial informatics. This Special Issue covers selected pieces of cutting-edge research works, ranging from low-level acquisition, to high-level analysis, processing, and manipulation of holographic information. The Special Issue also serves as a comprehensive review of existing state-of-the-art techniques in 3-D imaging and 3-D display, as well as broad insights into the future development of these disciplines. The Special Issue contains 25 papers in the field of holography, 3-D imaging, and 3-D display. All the papers underwent substantial peer review under the guidelines of Applied Sciences.
