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Author: Hans-Ferdinand Linskens
Publisher: Springer Science & Business Media
ISBN: 3642829511
Category : Science
Languages : en
Pages : 267
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Book Description
Modern Methods of Plant Analysis When the handbook Modern Methods of Plant Analysis was first introduced in 1954 the considerations were: 1. the dependence of scientific progress in biology on the improvement of existing and the introduction of new methods; 2. the difficulty in finding many new analytical methods in specialized journals which are normally not accessible to experimental plant biologists; 3. the fact that in the methods sections of papers the description of methods is frequently so compact, or even sometimes so incomplete that it is difficult to reproduce experiments. These considerations still stand today. The series was highly successful, seven volumes appearing between 1956 and 1964. Since there is still today a demand for the old series, the publisher has decided to resume publication of Modern Methods of Plant Analysis. It is hoped that the New Series will be just as acceptable to those working in plant sciences and related fields as the early volumes undoubtedly were. It is difficult to single out the major reasons for success of any publication, but we believe that the methods published in the first series were up-to-date at the time and presented in a way that made description, as applied to plant material, complete in itselfwith little need to consult other publications. Contributing authors have attempted to follow these guidelines in this New Series of volumes.
Author: Hans-Ferdinand Linskens
Publisher: Springer Science & Business Media
ISBN: 3642829511
Category : Science
Languages : en
Pages : 267
Get Book
Book Description
Modern Methods of Plant Analysis When the handbook Modern Methods of Plant Analysis was first introduced in 1954 the considerations were: 1. the dependence of scientific progress in biology on the improvement of existing and the introduction of new methods; 2. the difficulty in finding many new analytical methods in specialized journals which are normally not accessible to experimental plant biologists; 3. the fact that in the methods sections of papers the description of methods is frequently so compact, or even sometimes so incomplete that it is difficult to reproduce experiments. These considerations still stand today. The series was highly successful, seven volumes appearing between 1956 and 1964. Since there is still today a demand for the old series, the publisher has decided to resume publication of Modern Methods of Plant Analysis. It is hoped that the New Series will be just as acceptable to those working in plant sciences and related fields as the early volumes undoubtedly were. It is difficult to single out the major reasons for success of any publication, but we believe that the methods published in the first series were up-to-date at the time and presented in a way that made description, as applied to plant material, complete in itselfwith little need to consult other publications. Contributing authors have attempted to follow these guidelines in this New Series of volumes.
Author: Hans-Ferdinand Linskens
Publisher: Springer
ISBN: 9783540172437
Category : Science
Languages : en
Pages : 248
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Book Description
Modern Methods of Plant Analysis When the handbook Modern Methods of Plant Analysis was first introduced in 1954 the considerations were: 1. the dependence of scientific progress in biology on the improvement of existing and the introduction of new methods; 2. the difficulty in finding many new analytical methods in specialized journals which are normally not accessible to experimental plant biologists; 3. the fact that in the methods sections of papers the description of methods is frequently so compact, or even sometimes so incomplete that it is difficult to reproduce experiments. These considerations still stand today. The series was highly successful, seven volumes appearing between 1956 and 1964. Since there is still today a demand for the old series, the publisher has decided to resume publication of Modern Methods of Plant Analysis. It is hoped that the New Series will be just as acceptable to those working in plant sciences and related fields as the early volumes undoubtedly were. It is difficult to single out the major reasons for success of any publication, but we believe that the methods published in the first series were up-to-date at the time and presented in a way that made description, as applied to plant material, complete in itselfwith little need to consult other publications. Contributing authors have attempted to follow these guidelines in this New Series of volumes.
Author: Linskens
Publisher:
ISBN: 9788184891003
Category :
Languages : en
Pages :
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Book Description
Author: Monika Waksmundzka-Hajnos
Publisher: CRC Press
ISBN: 9781420092615
Category : Health & Fitness
Languages : en
Pages : 996
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Book Description
The powerful, efficient technique of high performance liquid chromatography (HPLC) is essential to the standardization of plant-based drugs, identification of plant material, and creation of new herbal medicines. Filling the void in this critical area, High Performance Liquid Chromatography in Phytochemical Analysis is the first book to give a comp
Author: Tomasz Tuzimski
Publisher: CRC Press
ISBN: 1498766544
Category : Science
Languages : en
Pages : 582
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Book Description
HPLC is the principal separation technique for identification of the pesticides in environmental samples and for quantitative analysis of analytes. At each stage of the HPLC procedure, the chromatographer should possess both the practical and theoretical skills required to perform HPLC experiments correctly and to obtain reliable, repeatable, and reproducible results. Developed to serve as a detailed practical guide, High Performance Liquid Chromatography in Pesticide Residue Analysis is a comprehensive source of information and training on state-of-the-art pesticide residue methods performed with the aid of HPLC. The book presents the pros and cons of HPLC as a flexible and versatile separation and analysis tool with multiple purposes and advantages in investigations of pesticides for food and plant drugs standardization, promotion of health, protection of new herbal medicines, and more.
Author: Alfred L. Yergey
Publisher: Springer Science & Business Media
ISBN: 148993605X
Category : Science
Languages : en
Pages : 310
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Book Description
This book is intended both to be an introduction to techniques and applications of liquid chromatography/mass spectrometry and to serve as a reference for future workers. When we undertook its writing, we chose not to cover the field, particularly applications, exhaustively. Rather we wished to produce a book that would be of use to people just beginning to use the technique as well as to more advanced practitioners. In this regard, we have sought to highlight techniques and applications that are of current importance, while not neglecting descriptions of approaches that may be of significance in the future. We hope that we have succeeded in this. At the same time we hope that the bibliography, with indexes classified by author and title, will make this book of value to those who may disagree with our emphasis. ACKNOWLEDGMENTS. One of us (C. G. E. ) wishes to acknowledge the en couragment of Professor J. A. McCloskey in undertaking this project. All four of us are grateful for the continuous and expert assistance of V. A. Edmonds in the preparation of the Bibliography. Alfred L. Y ergey Bethesda, Maryland Charles G. Edmonds Richland, Washington lvor A. S. Lewis London, England Marvin L. Vestal Houston, Texas v Contents 1. Introduction . . . . . . . . . . . . . . . . . . . . . . . . . 1 . . . . . . . . . . . 2. Direct Liquid Introduction Interfaces . . . . . . . . . . 5 . . . 2. 1. Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 2. 2. Operating Principles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 2. 3. Specific DLI Interfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 2. 3. 1. Capillary Inlets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 2. 3. 2. Diaphragm Interfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 2. 3. 3. Nebulizing Interfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Author: K. Biemann
Publisher: Springer
ISBN: 9783540028819
Category : Science
Languages : en
Pages : 536
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Book Description
123 phase and hence have no direct bearing on the retention time of solutes. However in gas-solid chromatography, a considerable quantity of the mobile phase may be adsorbed on the surface of the stationary adsorbent which diminishes the column's effective length and ability to retain solutes. In this respect helium has been found to be preferable to most other gases (GREENE and Roy, 1957) because it is adsorbed to the least extent. 3. Packed columns offer a considerable resistance to flow, which may create a pressure differential between inlet and outlet of sufficient magnitude to cause an unfavorable flow rate through a significant length of the column. A reduced inlet/outlet pressure ratio can be obtained by using light molecular weight gases toward which the column packing shows the greatest permeability. The flow rate of the mobile phase is normally adjusted by altering the column inlet pressure, for which purpose commercial pressure regulators of sufficient accuracy are available. Quantitative measurements of the flow rate can be made by a number of methods, including rotameters, orifice meters, soapfilm flow meters and displacement of water. The former two methods are the most con venient but the least accurate; moreover they create a back pressure and are temperature dependent whereas although the moving soap bubble is cumbersome to employ and unusable for continuous readings, it is preferred when the highest accuracy is required.
Author: Phyllis Brown
Publisher: Elsevier
ISBN: 0323160050
Category : Science
Languages : en
Pages : 214
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Book Description
High Pressure Liquid Chromatography: Biochemical and Biomedical Applications covers basic information on high pressure liquid chromatography in a simple and concise manner. It describes high pressure liquid chromatography, encompassing the method’s history and advantages. The book explains the instrumentations, experimental methods, peak identification, quantitation, and applications of high pressure liquid chromatography. It also discusses the pitfalls likely to be encountered in utilizing such method. This reference serves as an introductory book for all those who are unfamiliar with high pressure liquid chromatography. This book can also be used as a reference for those who are currently using the technique. It can also aid in promoting the use of high pressure liquid chromatography in all biochemical and biomedical researches.
Author: Chang Kee Lim
Publisher: World Scientific Publishing Company
ISBN:
Category : Chlorophyll
Languages : en
Pages : 252
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Book Description
The porphyrins, chlorophylls, bilins and related tetrapyrroles are vital for all living organisms. Natural and synthetic tetrapyrroles are used extensively in foods, cosmetics, biotechnology, pharmaceuticals, diagnostics and medicine. Methods for their separation and characterization therefore, have a very wide area of applications. Yet, there is a dearth of books dedicated to HPLC and HPLC/MS of tetrapyrroles. Lim addresses this problem admirably by providing practical HPLC and HPLC/MS protocols coupled with in-depth chromatographic and mass spectrometric reference data. These are invaluable in the analysis, identification and characterization of porphyrins, chlorophylls, bilins and other related compounds found in biological and clinical materials. HPLC method development and optimization for coupling to mass spectrometry are also described in rich detail. Sample preparation, and suggestions for avoiding procedural artifacts during extraction of clinical and biological samples are discussed. Clinical biochemists involved in biochemical diagnosis of human porphyrias will find this monograph assuredly helpful, as would analysts, biochemists and chemists involved in the separation, isolation and characterization of natural and synthetic tetrapyrroles. Undoubtedly, Lim has contributed a master-piece containing sufficient background material for beginners and up-to-date references for all researchers in the field.
Author: P. M. Dey
Publisher: Academic Press
ISBN: 0080984185
Category : Science
Languages : en
Pages : 608
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Book Description
Methods in Plant Biochemistry, Volume 1: Plant Phenolics reviews current knowledge about techniques used in the analysis of the biochemistry of plant polyphenols and their importance in the agricultural and food industries. It looks at the application of these techniques in the fractionation of cellular constituents, isolation of enzymes, electrophoretic separation of nucleic acids and proteins, and chromatographic identification of the intermediates and products of cellular metabolism. Organized into 15 chapters, this book opens with an overview of the general procedures and measurement of total phenolics, from detecting phenolic substances in crude plant extracts to determining which classes they belong to and the quantitative estimation of total phenol. The reader is introduced to the chemistry, structural variation, function, and distribution of each class of plant phenolics and, in a few cases where this is practicable, detailed listings of known derivatives are given. Most chapters focus on chromatographic separations and high performance liquid chromatography (HPLC), along with thin layer and paper Rf values with HPLC retention times and NMR spectroscopy. The book also outlines the procedures for the extraction, isolation, separation, and characterization of different classes of phenolic compounds, ranging from phenols and phenolic acids to phenylpropanoids, lignins, stilbenes and phenanthrenes, flavones and flavonols, chalcones and aurones, flavanoids, anthocyanins, biflavanoids, tannins, isoflavanoids, quinones, xanthones, and lichen substances. The book is a valuable resource for students, biochemists, and researchers in the plant sciences.
