Fundamental Laboratory Approaches for Biochemistry and Biotechnology PDF Download
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Author: Alexander J. Ninfa
Publisher: John Wiley & Sons
ISBN: 0470087668
Category : Science
Languages : en
Pages : 480
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Book Description
Ninfa/Ballou/Benore is a solid biochemistry lab manual, dedicated to developing research skills in students, allowing them to learn techniques and develop the organizational approaches necessary to conduct laboratory research. Ninfa/Ballou/Benore focuses on basic biochemistry laboratory techniques with a few molecular biology exercises, a reflection of most courses which concentrate on traditional biochemistry experiments and techniques. The manual also includes an introduction to ethics in the laboratory, uncommon in similar manuals. Most importantly, perhaps, is the authors' three-pronged approach to encouraging students to think like a research scientist: first, the authors introduce the scientific method and the hypothesis as a framework for developing conclusive experiments; second, the manual's experiments are designed to become increasingly complex in order to teach more advanced techniques and analysis; finally, gradually, the students are required to devise their own protocols. In this way, students and instructors are able to break away from a "cookbook" approach and to think and investigate for themselves. Suitable for lower-level and upper-level courses; Ninfa spans these courses and can also be used for some first-year graduate work.
Author: Alexander J. Ninfa
Publisher: John Wiley & Sons
ISBN: 0470087668
Category : Science
Languages : en
Pages : 480
Get Book
Book Description
Ninfa/Ballou/Benore is a solid biochemistry lab manual, dedicated to developing research skills in students, allowing them to learn techniques and develop the organizational approaches necessary to conduct laboratory research. Ninfa/Ballou/Benore focuses on basic biochemistry laboratory techniques with a few molecular biology exercises, a reflection of most courses which concentrate on traditional biochemistry experiments and techniques. The manual also includes an introduction to ethics in the laboratory, uncommon in similar manuals. Most importantly, perhaps, is the authors' three-pronged approach to encouraging students to think like a research scientist: first, the authors introduce the scientific method and the hypothesis as a framework for developing conclusive experiments; second, the manual's experiments are designed to become increasingly complex in order to teach more advanced techniques and analysis; finally, gradually, the students are required to devise their own protocols. In this way, students and instructors are able to break away from a "cookbook" approach and to think and investigate for themselves. Suitable for lower-level and upper-level courses; Ninfa spans these courses and can also be used for some first-year graduate work.
Author: Lisa A. Seidman
Publisher: CRC Press
ISBN: 1000480801
Category : Medical
Languages : en
Pages : 2036
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Book Description
Basic Laboratory Methods for Biotechnology, Third Edition is a versatile textbook that provides students with a solid foundation to pursue employment in the biotech industry and can later serve as a practical reference to ensure success at each stage in their career. The authors focus on basic principles and methods while skillfully including recent innovations and industry trends throughout. Fundamental laboratory skills are emphasized, and boxed content provides step by step laboratory method instructions for ease of reference at any point in the students’ progress. Worked through examples and practice problems and solutions assist student comprehension. Coverage includes safety practices and instructions on using common laboratory instruments. Key Features: Provides a valuable reference for laboratory professionals at all stages of their careers. Focuses on basic principles and methods to provide students with the knowledge needed to begin a career in the Biotechnology industry. Describes fundamental laboratory skills. Includes laboratory scenario-based questions that require students to write or discuss their answers to ensure they have mastered the chapter content. Updates reflect recent innovations and regulatory requirements to ensure students stay up to date. Tables, a detailed glossary, practice problems and solutions, case studies and anecdotes provide students with the tools needed to master the content.
Author: Timea Gerczei Fernandez
Publisher: Walter de Gruyter GmbH & Co KG
ISBN: 3110426250
Category : Science
Languages : en
Pages : 208
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Book Description
Biochemistry laboratory manual for undergraduates – an inquiry based approach by Gerczei and Pattison is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology while incorporating the blossoming field of bioinformatics. The novelty of this manual is the incorporation of a student-driven real real-life research project into the undergraduate curriculum. Since students test their own mutant design, even the most experienced students remain engaged with the process, while the less experienced ones get their first taste of biochemistry research. Inclusion of a research project does not entail a limitation: this manual includes all classic biochemistry techniques such as HPLC or enzyme kinetics and is complete with numerous problem sets relating to each topic.
Author: Friedrich Lottspeich
Publisher: John Wiley & Sons
ISBN: 3527694447
Category : Science
Languages : en
Pages : 450
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Book Description
Analytical methods are the essential enabling tools of the modern biosciences. This book presents a comprehensive introduction into these analytical methods, including their physical and chemical backgrounds, as well as a discussion of the strengths and weakness of each method. It covers all major techniques for the determination and experimental analysis of biological macromolecules, including proteins, carbohydrates, lipids and nucleic acids. The presentation includes frequent cross-references in order to highlight the many connections between different techniques. The book provides a bird's eye view of the entire subject and enables the reader to select the most appropriate method for any given bioanalytical challenge. This makes the book a handy resource for students and researchers in setting up and evaluating experimental research. The depth of the analysis and the comprehensive nature of the coverage mean that there is also a great deal of new material, even for experienced experimentalists. The following techniques are covered in detail: - Purification and determination of proteins - Measuring enzymatic activity - Microcalorimetry - Immunoassays, affinity chromatography and other immunological methods - Cross-linking, cleavage, and chemical modification of proteins - Light microscopy, electron microscopy and atomic force microscopy - Chromatographic and electrophoretic techniques - Protein sequence and composition analysis - Mass spectrometry methods - Measuring protein-protein interactions - Biosensors - NMR and EPR of biomolecules - Electron microscopy and X-ray structure analysis - Carbohydrate and lipid analysis - Analysis of posttranslational modifications - Isolation and determination of nucleic acids - DNA hybridization techniques - Polymerase chain reaction techniques - Protein sequence and composition analysis - DNA sequence and epigenetic modification analysis - Analysis of protein-nucleic acid interactions - Analysis of sequence data - Proteomics, metabolomics, peptidomics and toponomics - Chemical biology
Author: Jeffrey M. Becker
Publisher: Academic Press
ISBN: 0080528198
Category : Science
Languages : en
Pages : 261
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Book Description
The objectives of this Second Edition of Biotechnology: A Laboratory Course remain unchanged: to create a text that consists of a series of laboratory exercises that integrate molecular biology with protein biochemistry techniques while providing a continuum of experiments. The course begins with basic techniques and culminates in the utilization of previously acquired technical experience and experimental material. Two organisms, Sacchaomyces cerevisiae and Escherichia coli, a single plasmid, and a single enzyme are the experimental material, yet the procedures and principles demonstrated are widely applicable to other systems. This text will serve as an excellent aid in the establishment or instruction of introductory courses in the biological sciences. All exercises and appendixes have been updated Includes new exercises on: Polymerase chain reaction Beta-Galactosidase detection in yeast colonies Western blotting New procedures introduced for: Large-scale plasmid isolation Yeast transformation DNA quantitation New appendixes added, one of which provides details on accessing biological information sites on the Internet (World Wide Web) Use of non-radioactive materials and easy access to microbial cultures Laboratory exercises student tested for seven years
Author: Nicholas H. Acheson
Publisher: Wiley Global Education
ISBN: 1118213823
Category : Science
Languages : en
Pages : 528
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Book Description
Designed for students learning about viruses for the first time at the undergraduate or graduate level, Fundamentals of Molecular Virology is presented in a style which relates to today's students and professors. This book is also a valuable, up-to-date source of information for graduate students, postdoctoral fellows and research scientists working with viruses. Chapters contributed by prominent virologists were edited to conform to a clear and accessible style. The text provides a thorough presentation of basic and contemporary concepts in virology for a student's first exposure to the field.
Author: Lisa A. Seidman
Publisher: CRC Press
ISBN: 1000750116
Category : Medical
Languages : en
Pages : 444
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Book Description
Provides the basic laboratory skills and knowledge to pursue a career in biotechnology. Written by four biotechnology instructors with over 20 years of teaching experience, it incorporates instruction, exercises, and laboratory activities that the authors have been using and perfecting for years. These exercises and activities help students understand the fundamentals of working in a biotechnology laboratory. Building skills through an organized and systematic presentation of materials, procedures, and tasks, the manual explores overarching themes that relate to all biotechnology workplaces including forensic, clinical, quality control, environmental, and other testing laboratories. Features: • Provides clear instructions and step-by-step exercises to make learning the material easier for students. • Emphasizes fundamental laboratory skills that prepare students for the industry. • Builds students’ skills through an organized and systematic presentation of materials, procedures, and tasks. • Updates reflect recent innovations and regulatory requirements to ensure students stay up to date. • Supplies skills suitable for careers in forensic, clinical, quality control, environmental, and other testing laboratories.
Author: Timea Gerczei
Publisher: de Gruyter Open Poland
ISBN: 9783110411324
Category : Biochemistry
Languages : en
Pages : 0
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Book Description
Annotation Biochemistry Laboratory Manual for undergraduates is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology. Inclusion of a research project does not entail a limitation: this manual includes all classic biochemistry techniques such as HPLC or enzyme kinetics and is complete with numerous problem sets relating to each topic.
Author: Martin Holtzhauer
Publisher: Springer Science & Business Media
ISBN: 354032786X
Category : Science
Languages : en
Pages : 252
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Book Description
This book presents proven lab procedures and practical hints for research in analytical and preparative biochemistry, and offers convenient key data in numerous tables. Coverage includes quantitative methods; electrophoresis; chromatographic protocols; immunochemical protocols; centrifugation; and radioactivity. In additional chapters, tables offer quick access to a broad array of useful information, including SI units conversion factors; detergent, protein and nucleotide data; and the basic principles of statistics and enzyme and receptor kinetics are reviewed. This first English-language edition of a successful German-language manual is a valuable resource for students and working professionals in biochemistry, biotechnology and biomedical laboratories.
Author: Ravin Narain
Publisher: John Wiley & Sons
ISBN: 1118776372
Category : Science
Languages : en
Pages : 496
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Book Description
Explores bioconjugate properties and applications ofpolymers, dendrimers, lipids, nanoparticles, and nanotubes Bioconjugation has enabled breakthroughs across many areas ofindustry and biomedicine. With its emphasis on synthesis,properties and applications, this book enables readers tounderstand the connection between chemistry and the biologicalapplication of bioconjugated materials. Its detailed descriptionsof methods make it possible for researchers to fabricate and takefull advantage of bioconjugates for a broad range of applications.Moreover, the book sets the foundation for the development of newapplications, including assays, imaging, biosensors, drug delivery,and diagnostics. Chemistry of Bioconjugates features contributions from aninternational team of leading experts and pioneers in the field.These contributions reflect the authors’ firsthand laboratoryexperience as well as a thorough review of the current literature.The book’s six sections examine: General methods of bioconjugation Polymer bioconjugates Organic nanoparticle-based bioconjugates Inorganic nanomaterial bioconjugates, including metals andmetal oxides Cell-based, hydrogel/microgel, and glyco-bioconjugates Characterization, physico-(bio)chemical properties, andapplications of bioconjugates This comprehensive exploration of bioconjugates includesdiscussions of polymers, dendrimers, lipids, nanoparticles, andnanotubes. References at the end of each chapter serve as a gatewayto the most important original research findings and reviews in thefield. By drawing together and analyzing all the latest chemicalmethods and research findings on the physico-chemical andbiochemical properties of bioconjugates, Chemistry ofBioconjugates sheds new light on the significance and potentialof bioconjugation. The book is recommended for organic and polymerchemists, biochemists, biomaterial scientists, carbohydratechemists, biophysicists, bioengineers, and drug and gene deliveryscientists.
