Foreign Gene Expression in Fission Yeast: Schizosaccharomyces pombe PDF Download
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Author: Yuko Giga-Hama
Publisher: Springer Science & Business Media
ISBN: 3662034727
Category : Science
Languages : en
Pages : 179
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Book Description
Recombinant proteins, produced in cells or microorganisms, play an important role in basic research, medical science and the biotechnology industry. Many foreign gene expression systems using mammalian cells, E. coli, insect cells, yeast and other organisms as hosts have been developed. However, only recently has the expression system of Schizosaccharomyces pombe as a host become a subject of focus, as it is expected to be profitable for expressing foreign genes. The reason for this is that S. pombe is evolutionarily more advanced than other types of yeast. This book describes the use of S. pombe in basic research and industrial applications.
Author: Yuko Giga-Hama
Publisher: Springer Science & Business Media
ISBN: 3662034727
Category : Science
Languages : en
Pages : 179
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Book Description
Recombinant proteins, produced in cells or microorganisms, play an important role in basic research, medical science and the biotechnology industry. Many foreign gene expression systems using mammalian cells, E. coli, insect cells, yeast and other organisms as hosts have been developed. However, only recently has the expression system of Schizosaccharomyces pombe as a host become a subject of focus, as it is expected to be profitable for expressing foreign genes. The reason for this is that S. pombe is evolutionarily more advanced than other types of yeast. This book describes the use of S. pombe in basic research and industrial applications.
Author: Yuko Giga-Hama
Publisher:
ISBN: 9781570594670
Category : Recombinant proteins
Languages : en
Pages : 182
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Book Description
Author: Richard Egel
Publisher: Springer Science & Business Media
ISBN: 3662103605
Category : Science
Languages : en
Pages : 464
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Book Description
The fission yeast Schizosaccharomyces pombe is the favoured tool of many productive research groups throughout the world, serving as a useful model for fundamental principles and mechanisms, such as genome organization, differential gene regulation, cell-cycle control, signal transduction, or cellular morphogenesis. This book collates the current state of knowledge derived from molecular studies in this simple eukaryotic microorganism. The entire sequence of its genome has been completed, emphasizing the comparative value and model status of this yeast. The individual chapters, highlighting up-to-date views on prominent aspects of molecular organization, were written by active research scientists, presenting the results of their investigations to other workers in neighbouring fields. This book intends to serve the fission yeast community as a handy source of reference for years to come. It will also be of particular value to the ever-increasing number of researchers starting to look into fission yeast affairs for comparative reasons from other platforms of molecular genetics and cell biology.
Author: Michael N. Hall
Publisher:
ISBN: 9780879698744
Category :
Languages : en
Pages : 0
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Book Description
Yeast genetics began with Winge's 1935 studies of S. cerevisiae in Copenhagen, and afterwards was pursued by Lindegren in the U.S. and Ephrussi in France. Genetic studies in S. pombe were pioneered by Leupold in the 1940s in Switzerland. Within four decades, not without controversies, both yeast species were recognized as essential models in eukaryotic molecular cell biology. In this remarkable volume, Hall and Linder have assembled the reminiscences of many early investigators whose pioneering studies in the years before 1975 brought yeast biology to its current maturity. These illustrated essays about the science, the events and the personalities involved capture a fascinating era, in the informal style made famous by Phage and the Origins of Molecular Biology. This is a book that all scientists interested in the development of modern genetics and molecular biology should have on their shelves.
Author:
Publisher: Elsevier
ISBN: 0323140068
Category : Science
Languages : en
Pages : 488
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Book Description
This highly researched yeast, which represents a system used by cell biologists, geneticists and molecular biologists, has been given only minimal coverage in the literature. Its properties make it an excellent organism for DNA and related biotechnology reseach. This book, which is the first attempt to collate existing information in one source, will be an invaluable aid to those initiating projects with this organism.
Author: Martin Kollmar
Publisher: Humana Press
ISBN: 9781493977369
Category : Medical
Languages : en
Pages : 581
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Book Description
This volume explores databases containing genome-based data and genome-wide analyses. This book covers databases from all eukaryotic taxa, except plants. The chapters describe database contents and classic use-cases, which assist in accessing eukaryotic genomic data and encouraging comparative genomic research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, step-by-step, readily reproducible computational protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Eukaryotic Genomic Databases: Methods and Protocols is a valuable resource for geneticists and molecular biologists who are interested in the latest eukaryotic genomics data. The chapters 'PomBase: The Scientific Resource for Fission Yeast' and 'The Ensembl Genome Browser: Strategies for Accessing Eukaryotic Genome Data' are open access under a CC BY 4.0 license via link.springer.com.
Author: Mohammad Manjur Shah
Publisher: BoD – Books on Demand
ISBN: 9535121448
Category : Science
Languages : en
Pages : 144
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Book Description
Microbiology involves the study of microscopic living organisms. Most of them are unicellular and all the life processes are performed by a single cell. They are associated with the health and welfare of human beings. Among the biological sciences, microbiology has established itself a place in the current century. Microorganisms also provide experimental models in various research activities, and an answer to numerous fundamental questions in genetics / metabolism, cell form and function. This book is presented in six chapters comprising of two sections. The first section deals with Microbiology and Agriculture and the second section deals with Microbiology and Human Health. The book is expected to attract wide audience from various fields of biological sciences in general, and microbiologists in particular.
Author:
Publisher: Academic Press
ISBN: 9780123751737
Category : Science
Languages : en
Pages : 942
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Book Description
This fully updated edition of the bestselling three-part Methods in Enzymology series, Guide to Yeast Genetics and Molecular Cell Biology is specifically designed to meet the needs of graduate students, postdoctoral students, and researchers by providing all the up-to-date methods necessary to study genes in yeast. Procedures are included that enable newcomers to set up a yeast laboratory and to master basic manipulations. This volume serves as an essential reference for any beginning or experienced researcher in the field. Provides up-to-date methods necessary to study genes in yeast. Includes proceedures that enable newcomers to set up a yeast laboratory and to master basic manipulations. This volume serves as an essential reference for any beginning or experienced researcher in the field.
Author: Iain Hagan
Publisher:
ISBN: 9781621820819
Category : Schizosaccharomyces pombe
Languages : en
Pages : 0
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Book Description
Fission yeast are unicellular, rod-shaped fungi that divide by medial fission. Studies using fission yeast were instrumental in identifying fundamental mechanisms that govern cell division, differentiation, and epigenetics, to name but a few. Their rapid growth rate, genetic malleability, and similarities to more complex eukaryotes continue to make them excellent subjects for many biochemical, molecular, and cell biological studies. This laboratory manual provides an authoritative collection of core experimental procedures that underpin modern fission yeast research. The contributors describe basic methods for culturing and genetically manipulating fission yeast, synchronization strategies for probing the cell cycle, technologies for assessing proteins, metabolites, and cell wall constituents, imaging methods to visualize subcellular structures and dynamics, and protocols for investigating chromatin and nucleic acid metabolism. Modifications to techniques commonly used in related species (e.g., budding yeast) are noted, as are useful resources for fission yeast researchers, including various databases and repositories. The well-studied fission yeast Schizosaccharomyces pombe is the focus throughout, but the emerging model S. japonicus-a larger, dimorphic species with several desirable characteristics-is also covered. This manual is an important reference for existing fission yeast laboratories and will serve as an essential start-up guide for those working with fission yeast for the first time.
Author: National Research Council
Publisher: National Academies Press
ISBN: 0309070864
Category : Nature
Languages : en
Pages : 348
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Book Description
Scientific Frontiers in Developmental Toxicology and Risk Assessment reviews advances made during the last 10-15 years in fields such as developmental biology, molecular biology, and genetics. It describes a novel approach for how these advances might be used in combination with existing methodologies to further the understanding of mechanisms of developmental toxicity, to improve the assessment of chemicals for their ability to cause developmental toxicity, and to improve risk assessment for developmental defects. For example, based on the recent advances, even the smallest, simplest laboratory animals such as the fruit fly, roundworm, and zebrafish might be able to serve as developmental toxicological models for human biological systems. Use of such organisms might allow for rapid and inexpensive testing of large numbers of chemicals for their potential to cause developmental toxicity; presently, there are little or no developmental toxicity data available for the majority of natural and manufactured chemicals in use. This new approach to developmental toxicology and risk assessment will require simultaneous research on several fronts by experts from multiple scientific disciplines, including developmental toxicologists, developmental biologists, geneticists, epidemiologists, and biostatisticians.
