Fluorescence Microscopy and Fluorescent Probes PDF Download
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Author: J. Slavík
Publisher: Springer Science & Business Media
ISBN: 1489918663
Category : Science
Languages : en
Pages : 292
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Book Description
Fluorescence microscopy images can be easily integrated into current video and computer image processing systems. People like visual observation; they like to watch a television or computer screen, and fluorescence techniques are thus becoming more and more popular. Since true in vivo experiments are simple to perform, samples can be directly seen and there is always the possibility of manipulating the samples during the experiments; it is an ideal technique for biology and medicine. Images are obtained by a classical (now called wide-field) fluorescence microscope, a confocal scanning microscope, upright or inverted, with epifluorescence or transmission. Computerized image processing may improve definition, and remove glare and scattered light signal. It also makes it possible to compute ratio images (ratio imaging both in excitation and in emission) or lifetime imaging. Image analysis programs may supply a great deal of additional data of various types, starting with calculations of the number of fluorescent objects, their shapes, brightness, etc. Fluorescence microscopy data may be complemented by classical measurement in the cuvette yr by flow cytometry.
Author: J. Slavík
Publisher: Springer Science & Business Media
ISBN: 1489918663
Category : Science
Languages : en
Pages : 292
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Book Description
Fluorescence microscopy images can be easily integrated into current video and computer image processing systems. People like visual observation; they like to watch a television or computer screen, and fluorescence techniques are thus becoming more and more popular. Since true in vivo experiments are simple to perform, samples can be directly seen and there is always the possibility of manipulating the samples during the experiments; it is an ideal technique for biology and medicine. Images are obtained by a classical (now called wide-field) fluorescence microscope, a confocal scanning microscope, upright or inverted, with epifluorescence or transmission. Computerized image processing may improve definition, and remove glare and scattered light signal. It also makes it possible to compute ratio images (ratio imaging both in excitation and in emission) or lifetime imaging. Image analysis programs may supply a great deal of additional data of various types, starting with calculations of the number of fluorescent objects, their shapes, brightness, etc. Fluorescence microscopy data may be complemented by classical measurement in the cuvette yr by flow cytometry.
Author: W. T. Mason
Publisher: Elsevier
ISBN: 0080531776
Category : Science
Languages : en
Pages : 647
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Book Description
The use of fluorescent and luminescent probes to measure biological function has increased dramatically since publication of the First Edition due to their improved speed, safety, and power of analytical approach. This eagerly awaited Second Edition, also edited by Bill Mason, contains 19 new chapters and over two thirds new material, and is a must for all life scientists using optical probes. The contents include discussion of new optical methodologies for detection of proteins, DNA and other molecules, as well as probes for ions, receptors, cellular components, and gene expression. Emerging and advanced technologies for probe detection such as confocal laser scanning microscopy are also covered. This book will be essential for those embarking on work in the field or using new methods to enhance their research. TOPICS COVERED: * Single and multiphoton confocal microscopy * Applications of green fluorescent protein and chemiluminescent reporters to gene expression studies * Applications of new optical probes for imaging proteins in gels * Probes and detection technologies for imaging membrane potential in live cells * Use of optical probes to detect microorganisms * Raman and confocal raman microspectroscopy * Fluorescence lifetime imaging microscopy * Digital CCD cameras and their application in biological microscopy
Author: W. T. Mason
Publisher: Academic Press
ISBN:
Category : Nature
Languages : en
Pages : 702
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Book Description
The use of fluorescent and luminescent probes to measure biological function has increased dramatically since the publication of the first edition due to their improved speed, safety, and power of analytical approach. This eagerly awaited second edition contains 19 new chapters, over two-thirds new material, and is a must for all life scientists using optical probes.
Author: W. T. Mason
Publisher:
ISBN: 9780124778306
Category : Science
Languages : en
Pages : 433
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Book Description
A head-start into the practice of optical probe technology including the problems, the pitfalls and limitations likely to be encountered, the application to practical biological questions and the likely areas for future development.
Author: Jan Slavík
Publisher: Springer Science & Business Media
ISBN: 9780306460210
Category : Science
Languages : en
Pages : 298
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Book Description
The April 1997 conference held in Prague attracted the cream of primarily European and Russian researchers (with a handful from the US, primarily from the U. of Maryland School of Medicine) to the burgeoning biological and medical applications of innovative optical technology, particularly laser confocal fluorescence microscopy. Six invited review lectures illuminate these recent developments and their role in cell biology, including computer simulations of molecular behavior. The 34 other papers focus on the major themes of: new developments in fluorescence instrumentation; fluorescent probes; nucleic acid labels; and other fluorescent labels, markers, and fluorogenic substrates. Annotation copyrighted by Book News, Inc., Portland, OR
Author: Ulrich Kubitscheck
Publisher: John Wiley & Sons
ISBN: 3527338373
Category : Medical
Languages : en
Pages : 504
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Book Description
While there are many publications on the topic written by experts for experts, this text is specifically designed to allow advanced students and researchers with no background in physics to comprehend novel fluorescence microscopy techniques. This second edition features new chapters and a subsequent focus on super-resolution and single-molecule microscopy as well as an expanded introduction. Each chapter is written by a renowned expert in the field, and has been thoroughly revised to reflect the developments in recent years.
Author: J. Slavik
Publisher:
ISBN: 9781489918673
Category :
Languages : en
Pages : 328
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Book Description
Author: F. W. D. Rost
Publisher: Cambridge University Press
ISBN: 9780521410885
Category : Science
Languages : en
Pages : 486
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Book Description
Fluorescence microscopy is used for studying the distribution of substances which are present in very small amounts, for example in living cells. This magnificent new work provides comprehensive cover of all aspects of fluorescence microscopy - including instrumentation, applications, and the history of the technique. The first volume deals with instrumentation and techniques for fluorescence microscopy, and includes a chapter on quantification and scanning. The second volume deals with the applications of fluorescence microscopy in many fields. It includes information on autofluorescence, and an invaluable appendix provides an alphabetical list of fluorochromes, giving information concerning chemical structure, fluorescence properties, applications and suitable filter combinations.
Author: Chayan Kanti Nandi
Publisher: Frontiers Media SA
ISBN: 2889710718
Category : Science
Languages : en
Pages : 105
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Book Description
Author: Ruud Kraayenhof
Publisher: Springer Science & Business Media
ISBN: 3642560679
Category : Science
Languages : en
Pages : 401
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Book Description
The increased use of fluorescence techniques is greatly enhanced by the improved instrumentation pioneered by inventive scientists and now made available commercially by several high-tech companies. Moreover, the design and development of many new molecular probes with higher selectivity for specific microenvironmental properties has stimulated many new researchers to employ fluorescence techniques for solving their problems. This topic book, the second in his series, reflects this exciting scientific progress and deals, among others, with new approaches and new probes in fluorescence spectroscopy, single molecule fluorescence, applications in biomembrane and enzyme studies and imaging of living cells.
