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Author: Kurt Mendgen
Publisher: Springer Science & Business Media
ISBN: 3642758185
Category : Science
Languages : en
Pages : 342
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Book Description
Plants, fungi, and viruses were among the first biological objects studied with an electron microscope. One of the two first instruments built by Siemens was used by Helmut Ruska, a brother of Ernst Ruska, the pioneer in constructing electron microscopes. H. Ruska published numerous papers on different biological objects in 1939. In one of these, the pictures by G. A. Kausche, E. Pfankuch, and H. Ruska of tobacco mosaic virus opened a new age in microscopy. The main problem was then as it still is today, to obtain an appropriate preparation of the specimen for observation in the electron microscope. Beam damage and specimen thickness were the first obstacles to be met. L. Marton in Brussels not only built his own instrument, but also made considerable progress in specimen preparation by introducing the impregnation of samples with heavy metals to obtain useful contrast. His pictures of the bird nest orchid root impregnated with osmium were revolutionary when published in 1934. It is not the place here to recall the different techniques which were developed in the subsequent years to attain the modern knowledge on the fine structure of plant cells and of different plant pathogens. The tremendous progress obtained with tobacco mosaic virus is reflected in the chapter by M. Wurtz on the fine structure of viruses in this Volume. New cytochemical and immunological techniques considerably surpass the morphological information obtained from the pathogens, especially at the host-parasite interface.
Author: Kurt Mendgen
Publisher: Springer Science & Business Media
ISBN: 3642758185
Category : Science
Languages : en
Pages : 342
Get Book
Book Description
Plants, fungi, and viruses were among the first biological objects studied with an electron microscope. One of the two first instruments built by Siemens was used by Helmut Ruska, a brother of Ernst Ruska, the pioneer in constructing electron microscopes. H. Ruska published numerous papers on different biological objects in 1939. In one of these, the pictures by G. A. Kausche, E. Pfankuch, and H. Ruska of tobacco mosaic virus opened a new age in microscopy. The main problem was then as it still is today, to obtain an appropriate preparation of the specimen for observation in the electron microscope. Beam damage and specimen thickness were the first obstacles to be met. L. Marton in Brussels not only built his own instrument, but also made considerable progress in specimen preparation by introducing the impregnation of samples with heavy metals to obtain useful contrast. His pictures of the bird nest orchid root impregnated with osmium were revolutionary when published in 1934. It is not the place here to recall the different techniques which were developed in the subsequent years to attain the modern knowledge on the fine structure of plant cells and of different plant pathogens. The tremendous progress obtained with tobacco mosaic virus is reflected in the chapter by M. Wurtz on the fine structure of viruses in this Volume. New cytochemical and immunological techniques considerably surpass the morphological information obtained from the pathogens, especially at the host-parasite interface.
Author: Kurt Mendgen
Publisher: Springer
ISBN: 9783540527770
Category : Science
Languages : en
Pages : 336
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Book Description
Plants, fungi, and viruses were among the first biological objects studied with an electron microscope. One of the two first instruments built by Siemens was used by Helmut Ruska, a brother of Ernst Ruska, the pioneer in constructing electron microscopes. H. Ruska published numerous papers on different biological objects in 1939. In one of these, the pictures by G. A. Kausche, E. Pfankuch, and H. Ruska of tobacco mosaic virus opened a new age in microscopy. The main problem was then as it still is today, to obtain an appropriate preparation of the specimen for observation in the electron microscope. Beam damage and specimen thickness were the first obstacles to be met. L. Marton in Brussels not only built his own instrument, but also made considerable progress in specimen preparation by introducing the impregnation of samples with heavy metals to obtain useful contrast. His pictures of the bird nest orchid root impregnated with osmium were revolutionary when published in 1934. It is not the place here to recall the different techniques which were developed in the subsequent years to attain the modern knowledge on the fine structure of plant cells and of different plant pathogens. The tremendous progress obtained with tobacco mosaic virus is reflected in the chapter by M. Wurtz on the fine structure of viruses in this Volume. New cytochemical and immunological techniques considerably surpass the morphological information obtained from the pathogens, especially at the host-parasite interface.
Author: P. Narayanasamy
Publisher: CRC Press
ISBN: 9780824705916
Category : Science
Languages : en
Pages : 548
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Book Description
This work provides information on the detection, identification, and differentiation of all microbial plant pathogens - presenting modern protocols for rapid diagnosis of diseases based on biological, physical, chemical and molecular properties. It contains methods for the selection of disease-free seeds and vegetatively propagated planting materials and quarantine techniques for screening newly introduced plant materials.
Author: U. S. Singh
Publisher: CRC Press
ISBN: 1351430211
Category : Science
Languages : en
Pages : 836
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Book Description
Molecular Methods in Plant Pathology covers methods in phytopathology at the molecular level, including PCR techniques, electron microscopy, tissue culturing, and the cloning of disease-resistant genes. Phytopathologists, botanists, horticulturists, and anyone working in agriculture will find this a useful reference on biophysical, biochemical, biomolecular, and biotechnological methods.
Author: A. B. A. M. Baudoin
Publisher: Amer Phytopathological Society
ISBN:
Category : Science
Languages : en
Pages : 196
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Book Description
How to use this kit; Use and care of the light microscope; Recognition and terminology of disease symptoms and signs; Isolation of fungi and bacteria; Diagnosis of disease and proof of pathogenicity; Plant disease diagnosis in practice; Characteristics and structures of fungi; Root infection by phytophtora; Damping-off of seeds and seedlings; Soils fungistasis; Pathogen survival in soil; Biological control of soilborne plant pathogens by antagonistic fungi; Soilborne fungi; Population assessment of fusarium spp; Foliar infection by fungi; Dissemination of fungus spores and bacterial cells by wind and water; Fungicides for the control of foliar infection; Race identification of wheat leaf rust; Bacterial pathogens; Crown gall; Frost injury and ice nucleating bacteria; Viruses: mechanical transmission and host range; Insect transmission of plant viruses; Electron microscopy; Virus identification by serology; Study of plant-parasitic nematodes; Root-Knot nematodes: Symptoms, life stages, and control; Cyst nematodes; Foliar nematodes on begonia: Physiological spects of plant disease; Abiotic diseases; Disorders caused by higher plant.
Author: R. T. V. Fox
Publisher: Oxford University Press, USA
ISBN:
Category : Art
Languages : en
Pages : 236
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Book Description
The correct diagnosis of a plant disease is an essential prerequisite of its successful control. Diagnostic methods today include a number of traditional techniques such as direct observation or microscopy, as well as more recently developed procedures such as those based on immunological or nucleic acid analysis. This book provides a text reviewing the principles of all these techniques that will be suitable for advanced students of plant pathology who already have some basic background in the subject. The theories behind the methods are described and illustrated with numerous examples of plant diseases caused by fungi, bacteria and viruses, and the strengths and limitations of different techniques are compared. The book includes a number of colour photographs and will provide a very useful overview of this rapidly developing subject.
Author: Anthony William Robards
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 324
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Book Description
Author: R. E. F. Matthews
Publisher: CRC Press
ISBN: 1351088254
Category : Science
Languages : en
Pages : 465
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Book Description
Diagnosis of Plant Virus Diseases presents a comprehensive summary of methods currently available for the diagnosis of plant diseases caused by viruses and viroids. Up-to-date literature references are provided, brief accounts of the basis for particular methods are included, and detailed protocols are presented. Procedures discussed include the use of host plants, electron microscopy of in vitro preparations, serological procedures (especially forms of ELISA, monoclonal antibodies, serological specific electron microscopy, and immunoblotting), and nucleic acid hybridization procedures. Strategies are outlined for implicating virus-like pathogens as causes of diseases of unknown etiology, and problems involved in identifying complexes of transmission-dependent and helper viruses are discussed. The book will be extremely useful for phytopathologists, plant virologists, and research students and workers in plant virology laboratories and diagnostic plant pathology laboratories.
Author: Aiming Wang
Publisher: Humana
ISBN: 9781071618370
Category : Science
Languages : en
Pages : 0
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Book Description
This volume discusses traditional and current techniques that are successfully used to diagnose plant viruses and study molecular plant-virus interactions. The chapters in this book cover topics such as in vivo detection of double-stranded RNA, developing rice mutant using CRISPR-Cas9-based technology, protein-protein interaction assays, purification and transfection of protoplasts, protocols for gene silencing, and transmission electron microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Plant Virology: Methods and Protocols is a valuable resource for plant pathologists, microbiologists, virologists, graduate students, and teachers who are interested in learning more about the developments in plant virology research.
Author: Chelston W. D. Brathwaite
Publisher: IICA
ISBN: 9789290390138
Category : Plant deseases
Languages : en
Pages : 56
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Book Description
The diagnosis of plant disease; The microscope; The autoclave; The preparation of media for fungal and bacterial growth; Detection of fungal pathogens in infected plant tissues; Detection of bacterial pathogens in infected tissues; Koch's postulates; The diagnosis of a nematode prblem; Viruses and plant virus diseases; Mycoplasma as agents of plant disease.
